I am currently working with 30-40 year old celloidin embedded tissue that I have sectioned myself. I have used a myelin stain of a modified Weigert's and am trying to get a Nissl stain to work with no luck.
The 40 micron sections are being kept in 70% etoh in individual series jars. Weigert's stain works perfectly however when I attempt to stain for Nissl substance the sections turn out blotchy, uneven and faded as if the background is the only thing that is holding the stain. I have tried soaking the sections in 50% etoh for at least an hour before going into dH20 for 2 hours up to 2 days. Nothing has worked. Does anyone have any ideas that might help? Something to dissolve a possible mordant? My only other option is to dissolve the celloidin in a 50/50 mixture of absolute etoh and ether which could damage the tissue/structures within. Crystal V. Saint Louis University Center for Anatomical Science Education _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet