Try decreasing the time in periodic acid. Basement membrane will over oxidize faster than the fungus. Some docs like the over-oxidation to high-light the fungus.
"Martin, Erin" <erin.mar...@ucsf.edu> Sent by: histonet-boun...@lists.utsouthwestern.edu 03/15/2011 07:19 AM To histonet <histonet@lists.utsouthwestern.edu> cc Subject [Histonet] PAS for basement membrane Hi all, I'm having a problem with my PAS stain. My pathologist says that glycogen and fungus stain beautifully but basement membranes are pale. We are using 0.5% periodic acid for 10 minutes and schiff's for 25 minutes followed by 5 min rinse in warm/hot water. Any thoughts? Thank you in advance! Thanks Erin Erin Martin, Histology Supervisor UCSF Department of Dermatopathology 415-353-7248 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
