Make the periodic acid fresh that day. Use 3 2 minute washes in 0.5% sodium meta-bisulfite instead of water.
Geoff On 3/15/2011 10:16 AM, Martin, Erin wrote:
Hi all, I'm having a problem with my PAS stain. My pathologist says that glycogen and fungus stain beautifully but basement membranes are pale. We are using 0.5% periodic acid for 10 minutes and schiff's for 25 minutes followed by 5 min rinse in warm/hot water. Any thoughts? Thank you in advance! Thanks Erin Erin Martin, Histology Supervisor UCSF Department of Dermatopathology 415-353-7248 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
-- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcaul...@umdnj.edu ********************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
