My question is directed specifically to veterinary histologists or histologists who also do a fair amount of animal processing. We are having a terrible time processing pig fat. We had problems previously, but thought we had solved them. This latest project (pig skin with a lot of fat attached) came out awful. The fat was not adequately processed: couldn't section it, it just crumbled. In the block, it appears white and crumbly. The funny thing is some blocks came out all right, but most didn't.
PLEASE help! Let me know how you process your animal fat (sp. Pig)! Is there a size issue (we trim it if it is greater than 5mm)? We have gotten help from the histonet before and instituted these suggestions (i.e. let sit in formalin for 48 hours; put in cassettes in a 60 degree oven for 1-2 hours and then cool to room temp. Then process using a 1 hour program). Any help would be appreciated. Thanks! Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 55 Fruit Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherw...@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet