I concur with the others. For about 6 years I've made it from powder but
the toxicity risk bothered me until I realized how safely and cheaply you
can just buy the same stuff. We get a discount through our stockroom on a
4L bottle of 10% Buffered Formalin from Fisher (SF100-4) for $28.
I started getting neutral buffered formalin instead from Fisher.
--On Monday, March 21, 2011 11:25 AM -0500 Montina Van Meter
<montina.vanme...@pbrc.edu> wrote:
Nick,
I too have tried to limit my exposure after making it from scratch for
30+ years. I currently purchase it from USB Corp. located in Cleveland
Ohio. They have been acquired by Affymetrix and you will be directed to
that website when you type in www.usbweb.com.
Cat.# 19943 1LT Paraformaldehyde Solution, 4% in PBS, 1L $48.00
Regards,
Tina
Montina J. Van Meter
Lab Manager
Autonomic Neuroscience
225-763-2564
vanme...@pbrc.edu
-----Original Message-----
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Madary,
Joseph
Sent: Monday, March 21, 2011 10:27 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] PFA preparation
Anyone have a decent method to prepare paraformaldehyde. I have the
powder and the NOAH, hood, heat source but forgot the method.
Nick Madary, HT/HTL(ASCP)QIHC
Histology Mgr, Medimmune
301.398.6360(lab), 4745(vm),9745(fax)
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