We also purchase a 4L cubitainer of 10% buffered formalin from Fisher. -----Original Message----- From: [email protected] [mailto:[email protected]] On Behalf Of Merced Leiker Sent: Monday, March 21, 2011 12:44 PM To: Montina Van Meter; Madary, Joseph; [email protected] Subject: RE: [Histonet] PFA preparation
I concur with the others. For about 6 years I've made it from powder but the toxicity risk bothered me until I realized how safely and cheaply you can just buy the same stuff. We get a discount through our stockroom on a 4L bottle of 10% Buffered Formalin from Fisher (SF100-4) for $28. I started getting neutral buffered formalin instead from Fisher. --On Monday, March 21, 2011 11:25 AM -0500 Montina Van Meter <[email protected]> wrote: > Nick, > > I too have tried to limit my exposure after making it from scratch for > 30+ years. I currently purchase it from USB Corp. located in Cleveland > Ohio. They have been acquired by Affymetrix and you will be directed to > that website when you type in www.usbweb.com. > > Cat.# 19943 1LT Paraformaldehyde Solution, 4% in PBS, 1L $48.00 > > > Regards, > Tina > > > > > > Montina J. Van Meter > Lab Manager > Autonomic Neuroscience > 225-763-2564 > [email protected] > > > > > -----Original Message----- > From: [email protected] > [mailto:[email protected]] On Behalf Of Madary, > Joseph > Sent: Monday, March 21, 2011 10:27 AM > To: [email protected] > Subject: [Histonet] PFA preparation > > Anyone have a decent method to prepare paraformaldehyde. I have the > powder and the NOAH, hood, heat source but forgot the method. > > > Nick Madary, HT/HTL(ASCP)QIHC > > Histology Mgr, Medimmune > > 301.398.6360(lab), 4745(vm),9745(fax) > > > "To the extent this electronic communication or any of its attachments > contain information that is not in the public domain, such information > is considered by MedImmune to be confidential and proprietary, and > expected to be used only by the individual(s) for whom it is intended. > If you have received this electronic communication in error, please > reply to the sender advising of the error in transmission and delete the > original message and any accompanying documents from your system > immediately, without copying, reviewing or otherwise using them for any > purpose. Thank you for your cooperation." > > > To the extent this electronic communication or any of its attachments > contain information that is not in the public domain, such information > is considered by MedImmune to be confidential and proprietary. This > communication is expected to be read and/or used only by the > individual(s) for whom it is intended. If you have received this > electronic communication in error, please reply to the sender advising > of the error in transmission and delete the original message and any > accompanying documents from your system immediately, without copying, > reviewing or otherwise using them for any purpose. Thank you for your > cooperation. > _______________________________________________ > Histonet mailing list > [email protected] > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > [email protected] > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II 354 Biomedical Research Building School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
