Having read all the other comments, here's my 2.5 pence worth! Have you tried a background reducing antibody diluent? You can get these from DAKO or Menarini Diagnostics: http://www.dako.com/uk/ar38/p107410/prod_products.htm.
Or MP-905-25 / MP-905-100, depending on the volume you require, from Menarini Diagnostics, website http://www.dako.com/uk/ar38/p107410/prod_products.htm. I found these useful for rabbit polyclonals. Good luck Margaret Miss Margaret Blount Histology Manager Metabolic Research Laboratories Level 4 Institute of Metabolic Science Box 289, Addenbrooke's Hospital Hills Road, Cambridge, CB2 0QQ Tel 01223 769061/336079 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of sgoe...@mirnarx.com Sent: 22 March 2011 21:04 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Stupid Rabbit primaries! So I haven't had to deal with rabbit polyclonal primaries in a long time because I remember how much the background sucks with them. Unfortunately the only available antibody is a rabbit polyclonal. Does anyone have any suggestions for how to eliminate the background? I have diluted almost to the point of the antigens not showing! Thanks guys and gals!! Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet