Hello, We do have a successful way to transfer tissue sections. It is using Mount Quik liquid coverslip. We purchase this from Newcomber Supply. Are you doing IHC testing? 1. H&E sections first (so they are visible)(for IHC, retrieval will fade the stain anyways) 2. After the stain, run slide to Xylene 3. Cover entire slide with layer of Mount Quik 4. Bake horizontal in 60 degree oven for 1 hour 5. Bake vertical for one hour 6. Rinse slide in running warm water until the mount quik and tissue lift from the slide 7. Divide the tissue as need, place portions on plus slide 8. Bake horizontal for 1 hour 9. Bake vertical for one hour 10. Dissolve off mount quik with several 10 minute steps in Xylene. No visible residual should be left 11. Carry out testing
Hope this helps Melissa -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, April 13, 2011 3:54 PM To: histonet@lists.utsouthwestern.edu; Jeanine (CDC/OID/NCEZID)Bartlett Subject: Re: [Histonet] Tissue transfer "Mission impossible" (successfully, you can always ruin them)! It is better to do the staining after a prolonged oven heating and do it delicately. René J. --- On Wed, 4/13/11, Bartlett, Jeanine (CDC/OID/NCEZID) <j...@cdc.gov> wrote: From: Bartlett, Jeanine (CDC/OID/NCEZID) <j...@cdc.gov> Subject: [Histonet] Tissue transfer To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Date: Wednesday, April 13, 2011, 1:06 PM Hello all, We have some unstained slides we received from outside the U.S. on non-charged/un-coated slides. We would like to transfer these sections to charged slides before proceeding with testing. Any suggestions? Thanks! Jeanine Bartlett, BS, HT(ASCP)QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartl...@cdc.hhs.gov _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
