The endogenous peroxidase has to be blocked (quenching others call this step) only before the procedure because the idea is to avoid the interference of the peroxidase with it. René J.
--- On Thu, 4/21/11, Emily Sours <talulahg...@gmail.com> wrote: From: Emily Sours <talulahg...@gmail.com> Subject: [Histonet] endogenous peroxidases To: histonet@lists.utsouthwestern.edu Date: Thursday, April 21, 2011, 4:32 PM Hello I know I can block endogenous peroxidases in paraffin sections before antigen retrieval or after it, but which is better? Does it make a difference? Also, since our H2O2 is 30%, do I need to account for the water in it when I'm diluting it in 100% MeOH? (I was going to use a 0.3% H2O2 in MeOH wash for 30 minutes) I'm not even sure how I would account for it, I just thought it might affect the MeOH since it wouldn't be anhydrous anymore. Emily ps put your hand to the screen for an internet high five!! it's national high five day! A great book should leave you with many experiences, and slightly exhausted. You should live several lives while reading it. -William Styron _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
