So I have been seeing from this post that people block prior to HIER? I have always done it as the first step after for 10 minutes (I use the DAKO enzyme blocker). Is this wrong? Thanks
Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Akemi Allison Sent: Friday, April 22, 2011 12:42 PM To: Hobbs, Carl Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] endogenous peroxidases Make sure you use a fairly fresh bottle of H2O2. (no older than 6 months). The stock bottle degrades after it is opened. I have seen people using an older bottle of H2O2 and the results are sub-optimal. Akemi Akemi Allison BS, HT (ASCP) HTL Director Phoenix Lab Consulting Tele: 408.335.9994 E-Mail: akemiat3...@yahoo.com On Apr 22, 2011, at 10:27 AM, Hobbs, Carl wrote: > > Gimme 5! > > Block before or after Ag retrieval. > If it's Pwax sections make a 1/10 dilution of 100vols ( ~30%) H2O2 > in Dist.water. > Block for 15mins. > Methanol is not neccessary...imho. > Carl > > > > Carl Hobbs > Histology Manager > Wolfson CARD > School of Biomedical Sciences > Kings College London > Guys Campus > London > SE1 1UL > Tel: 020 78486813 > Fax: 020 78486816 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet