We receive these specimens fresh and hand-process through 100% alcohol, xylene, and paraffin. The crystals are water soluble, so you need to avoid any aqueous solutions. We cut one slide for routine H&E staining and one slide that we just deparaffinize in xylene and then coverslip directly out of that xylene. The pathologist looks at the unstained slide with a polarizing lens. If the tissue gets processed routinely (through formalin and the graded alcohols), we still cut the two slides and sometimes the crystals are still there. Laurie Colbert
-----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Diana McCaig Sent: Monday, May 16, 2011 5:47 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] uric acid crystals in tissue for gout Does anyone have a method for identifying uric acid crystals in gout on a tissue sample. Diana _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
