And that is why I never let you use my knives. Tom
-----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill Sent: Monday, June 20, 2011 10:31 AM To: histot...@imagesbyhopper.com; Paula Sicurello Cc: histonet@lists.utsouthwestern.edu; Amos Brooks Subject: RE: [Histonet] Retirement You had COTTON in your pipettes? We used hollowed out reeds with a bit of papyrus in one end. No calibration.... But plenty accurate enough for histo in those days. However, there are a few things I do not miss from back in da' day. Hand-stropping a knife for an hour, only to have it nicked by a staple in the next hour. -Bill -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histot...@imagesbyhopper.com Sent: Sunday, June 19, 2011 7:48 PM To: Paula Sicurello Cc: histonet@lists.utsouthwestern.edu; Amos Brooks Subject: Re: [Histonet] Retirement And remember mouth pipeting? "oops, that's the cotton..." Eating and smoking in the lab was the norm. Our alcohol had the tax stamp on it! :o) Michelle On Jun 19, 2011, at 4:19 PM, Paula Sicurello <pat...@gmail.com> wrote: > Sheesh! We used to have people smoke while working with propylene oxide. > > Eating in your control pigs was part of the benefit of being a > graduate student to save on grocery money. > > Film? My TEM used glass plates. > > Lab mates used to routinely drink diet coke and 100% ethanol on Fridays. > > Wearing closed toed shoes was for wimps, you were just fast if you > dropped a steel wedge blade. > > We even wrote using the entire word and proper grammar, none of this > acronym stuff for us. > > Retirement? What's that? > > Paula :-) > > On Sun, Jun 19, 2011 at 12:31 PM, Amos Brooks <amosbro...@gmail.com> wrote: >> Agarose Gels! >> ... Listen you whipersnapper Agarose is the easy way out. When I >> learned it we used to have to make up our own polyacrylamide gels. >> That was after having to walk to work up hill both ways in 30 feet of snow! >> >> (No nearer retirement) >> Crotchety Amos >> >> >> >> Message: 7 >> Date: Fri, 17 Jun 2011 13:24:12 -0400 >> From: Emily Sours <talulahg...@gmail.com> >> Subject: Re: [Histonet] Retirement >> To: histonet@lists.utsouthwestern.edu >> Message-ID: <banlktinejxtxyop-byfweuxn3yw-ff3...@mail.gmail.com> >> Content-Type: text/plain; charset=UTF-8 >> >> Retirement? I think by the time I get to that point, social security >> will have run out. >> Then again, technology will be so advanced, I can tell stories about >> the old days, where I logged on to the bbs by modem to post messages >> to my friends and typed in my own html coding. >> We didn't have google when I was young!! Our cameras used film! And >> you couldn't see how bad your pictures were until you developed that film!! >> There was no PCR to sequence your DNA, you ran an agarose gel and >> hoped for the best!! You could drink the 100% ethanol, there was no >> denaturing! (okay that was before my time) You could smoke in the lab >> while you sectioned without gloves!! (okay that was too) >> >> Emily >> >> A great book should leave you with many experiences, and slightly exhausted. >> You should live several lives while reading it. >> -William Styron >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet