Hi there, I am very new to basic sciences research and am planning an experiment to quantify and compare adipose tissue macrophage infiltration in sheep model using immunohistochemistry. I am specifically interested in CD11c as a marker of M1 activation of Macrophages. I have been advised that it is not easy and previous effort by my predecessors have not been very successful in this! >From the general google, histonet/histosearch and pubmed search. I have made a >list of potential candidates:
1. Clones PG M1 and Clone KP1 (Source : Histonet : http://www.histosearch.com/histonet/Nov03A/RE.HistonetSeepmacrophage.html) 2. EMB11, a mouse anti-human CD68 mAb (Dako, Carpenteria, CA) (source: published paper: http://www.ncbi.nlm.nih.gov/pubmed/16460804 ) 1. Another CD68 antibody Source: http://www.ingentaconnect.com/content/ocean/ajra/2011/00000025/00000002/art00024) 1. Iba1 (ionized calcium binding adaptor molecule 1) is a 17-kDa EF hand protein that is specifically expressed in macrophages/microglia and is upregulated during the activation of these cells.[Wikipedia] (source: I do not remember where I found this one from but Abcam has several antibodies none of which are predicted for sheep) I am writing to ask for suggestions about which one to go for and any other tips about searching for antibodies or performing IHC in paraffin fixed adipose tissue/liver. Any ideas will be gratefully appreciated as I am very new and isolated in this aspect. Best wishes Vivek Dr Vivek Saroha Clinical lecturer in Child health University of Nottingham This message and any attachment are intended solely for the addressee and may contain confidential information. If you have received this message in error, please send it back to me, and immediately delete it. Please do not use, copy or disclose the information contained in this message or in any attachment. Any views or opinions expressed by the author of this email do not necessarily reflect the views of the University of Nottingham. This message has been checked for viruses but the contents of an attachment may still contain software viruses which could damage your computer system: you are advised to perform your own checks. Email communications with the University of Nottingham may be monitored as permitted by UK legislation._______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
