CD68 - clone KP1 has not worked on sheep (also not dogs, cows, horses, and
chickens) in my experience. I have found it to stain primates well (and
rare cells in pigs and cats).
Jan Shivers
Senior Scientist
Histology/IHC/EM Section Head
Pathology Teaching Program
University of Minnesota
Veterinary Diagnostic Laboratory
1333 Gortner Ave.
St. Paul, MN 55108
612-624-7297
shive...@umn.edu
(Confidentiality Notice: This message, together with any attachments, is
intended only for the use of the individual or entity to which it is
addressed and may contain confidential or privileged information. If you
think you have received this message in error, please advise the sender and
then delete this message and any attachments immediately.)
----- Original Message -----
From: "Vivek Saroha" <vivek.sar...@nottingham.ac.uk>
To: <histonet@lists.utsouthwestern.edu>
Sent: Friday, June 24, 2011 4:05 AM
Subject: [Histonet] Macrophage marker in sheep for IHC
Hi there,
I am very new to basic sciences research and am planning an experiment to
quantify and compare adipose tissue macrophage infiltration in sheep model
using immunohistochemistry. I am specifically interested in CD11c as a
marker of M1 activation of Macrophages. I have been advised that it is not
easy and previous effort by my predecessors have not been very successful in
this!
From the general google, histonet/histosearch and pubmed search. I have
made a list of potential candidates:
1. Clones PG M1 and Clone KP1
(Source : Histonet :
http://www.histosearch.com/histonet/Nov03A/RE.HistonetSeepmacrophage.html)
2. EMB11, a mouse anti-human CD68 mAb (Dako, Carpenteria, CA)
(source: published paper: http://www.ncbi.nlm.nih.gov/pubmed/16460804 )
1. Another CD68 antibody
Source:
http://www.ingentaconnect.com/content/ocean/ajra/2011/00000025/00000002/art00024)
1. Iba1 (ionized calcium binding adaptor molecule 1) is a 17-kDa EF hand
protein that is specifically expressed in macrophages/microglia and is
upregulated during the activation of these cells.[Wikipedia]
(source: I do not remember where I found this one from but Abcam has several
antibodies none of which are predicted for sheep)
I am writing to ask for suggestions about which one to go for and any other
tips about searching for antibodies or performing IHC in paraffin fixed
adipose tissue/liver.
Any ideas will be gratefully appreciated as I am very new and isolated in
this aspect.
Best wishes
Vivek
Dr Vivek Saroha
Clinical lecturer in Child health
University of Nottingham
This message and any attachment are intended solely for the addressee and
may contain confidential information. If you have received this message in
error, please send it back to me, and immediately delete it. Please do not
use, copy or disclose the information contained in this message or in any
attachment. Any views or opinions expressed by the author of this email do
not necessarily reflect the views of the University of Nottingham.
This message has been checked for viruses but the contents of an attachment
may still contain software viruses which could damage your computer system:
you are advised to perform your own checks. Email communications with the
University of Nottingham may be monitored as permitted by UK
legislation._______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
