Are you using synthetic xylene in your H&E/COVER SLIPPING protocol. If yes, you might want to consider ending your protocol with the real thing (XYLENE). I had the same situation until we switched to real XYLENE. No more H&E fading.
THANK YOU, PATTI RUBEN-NELSON H.T.(ASCP) PNP LABORATORY CONSULTANTS SUPERVISOR/DGC P.O. BOX 412 CABAZON, CA. 92230 cell (909) 841-9761 nelsonr...@verizon.net ________________________________ From: "amitapan...@torrentpharma.com" <amitapan...@torrentpharma.com> To: Histonet <histonet@lists.utsouthwestern.edu>; histonet-boun...@lists.utsouthwestern.edu; histonet <histonet-requ...@lists.utsouthwestern.edu> Sent: Wed, July 6, 2011 9:22:38 PM Subject: [Histonet] Routine H & E stain, Hello Histonetters, I am from toxicopathology lab where we perform H&E on rat tissues and store these slides for 10 long years. We use self lab prepare Harris hematoxylin (Hematoxylin crystal+Alcohol+ Ammonium alum +D/W and Mercuric oxide for ripening) and 1% aqueous eosin. The staining result is good (Purplish pink look stained slide) on all tissue, but our observation is after 5-6 months time this get faded and become towards pinkish type though we can observe the slide. We want to retain its fresh stained color. Please suggest me how to keep stable stain for longer period or do you suggest to switch to any other type of haematoxylin - prepared or commercially available? Looking forward for your feed back. Amita _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
