Amita, We are a neurotoxicology lab that also provides histology services for other researchers inside & outside Rutgers, and we use Gill's #3 hematoxylin for our routine H&Es on mouse and rat tissues. No fading that I have noticed in all the years that I have been here. We get ours from Thermo Fisher, or whatever they call themselves nowadays. :o)
Good luck, Kathleen Roberts Principal Lab Technician Neurotoxicology Labs Molecular Pathology Facility Core Dept of Pharmacology & Toxicology Rutgers, the State University of NJ 41 B Gordon Road Piscataway, NJ 08854 (732) 445-6914 > Hello Histonetters, > > I am from toxicopathology lab where we perform H&E on rat tissues and > store these slides for 10 long years. > > We use self lab prepare Harris hematoxylin (Hematoxylin crystal+Alcohol+ > Ammonium alum +D/W and Mercuric oxide for ripening) and 1% aqueous eosin. > The staining result is good (Purplish pink look stained slide) on all > tissue, but our observation is after 5-6 months time this get faded and > become towards pinkish type though we can observe the slide. > We want to retain its fresh stained color. > > Please suggest me how to keep stable stain for longer period or do you > suggest to switch to any other type of haematoxylin - prepared or > commercially available? > > Looking forward for your feed back. > Amita > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet