Does anyone else leave out the cell conditioning step?
-----Original Message----- From: Yahoo [mailto:kim.tourn...@yahoo.com] Sent: Tuesday, August 16, 2011 7:41 AM To: Sheila Fonner Subject: Re: [Histonet] IHC on Frozen Tissue I would leave out the pretreatment step as well i.e. Enzyme, CC1/CC2. Sent from the iPhone of Kim Tournear On Aug 16, 2011, at 6:03 AM, "Sheila Fonner" <sfon...@labpath.com> wrote: > Thank you. > > > -----Original Message----- > From: Mierow, Brett T. [mailto:brett.mie...@essentiahealth.org] > Sent: Tuesday, August 16, 2011 7:02 AM > To: Sheila Fonner > Subject: RE: [Histonet] IHC on Frozen Tissue > > You are correct, leave out the depar. step. > > > Brett Mierow, HT (ASCP) > Histology specialist > Essentia Health > SMDC Laboratory > 407 East 3rd Street, Duluth, MN 54880 > (218) 786-4510 | brett.mie...@essentiahealth.org > > -----Original Message----- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sheila > Fonner > Sent: Tuesday, August 16, 2011 5:41 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] IHC on Frozen Tissue > > Good morning everyone, > > > > I was wondering if anyone could help me with a protocol change for the > Ventana Ultra. I have a pathologist that is requesting an HSVI, HSVll, > and Zoster Virus on frozen tissue that was received for IF studies. I > know it's not Monday, but it is early, and I was hoping for some > information about how to change the protocol for frozen vs. paraffin > tissue. Do I just need to delete the depar. step and leave everything > else the same? I appreciate any help you could give. > > > > Thanks, > > Sheila > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
