Rich,
Isn't the cell conditioning (retrieval) mostly done for tissues that have been in formalin? If the tissue is fresh (frozen), it has not seen formalin. That is the reason I questioned the cell conditioning step. Thank you for your help. Sheila From: Richard Yeo [mailto:r...@wchosp.org] Sent: Tuesday, August 16, 2011 7:54 AM To: Sheila Fonner Subject: RE: [Histonet] IHC on Frozen Tissue Sheila, The cell conditioning step is for retrieval. It will depend on what antibody you are going to run. If you use it for Depar. you will also use it wiith-out. Rich Y _____ From: histonet-boun...@lists.utsouthwestern.edu on behalf of Sheila Fonner Sent: Tue 8/16/2011 7:44 AM To: 'Yahoo'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] IHC on Frozen Tissue Does anyone else leave out the cell conditioning step? -----Original Message----- From: Yahoo [mailto:kim.tourn...@yahoo.com] Sent: Tuesday, August 16, 2011 7:41 AM To: Sheila Fonner Subject: Re: [Histonet] IHC on Frozen Tissue I would leave out the pretreatment step as well i.e. Enzyme, CC1/CC2. Sent from the iPhone of Kim Tournear On Aug 16, 2011, at 6:03 AM, "Sheila Fonner" <sfon...@labpath.com> wrote: > Thank you. > > > -----Original Message----- > From: Mierow, Brett T. [mailto:brett.mie...@essentiahealth.org] > Sent: Tuesday, August 16, 2011 7:02 AM > To: Sheila Fonner > Subject: RE: [Histonet] IHC on Frozen Tissue > > You are correct, leave out the depar. step. > > > Brett Mierow, HT (ASCP) > Histology specialist > Essentia Health > SMDC Laboratory > 407 East 3rd Street, Duluth, MN 54880 > (218) 786-4510 | brett.mie...@essentiahealth.org > > -----Original Message----- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sheila > Fonner > Sent: Tuesday, August 16, 2011 5:41 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] IHC on Frozen Tissue > > Good morning everyone, > > > > I was wondering if anyone could help me with a protocol change for the > Ventana Ultra. I have a pathologist that is requesting an HSVI, HSVll, > and Zoster Virus on frozen tissue that was received for IF studies. I > know it's not Monday, but it is early, and I was hoping for some > information about how to change the protocol for frozen vs. paraffin > tissue. Do I just need to delete the depar. step and leave everything > else the same? I appreciate any help you could give. > > > > Thanks, > > Sheila > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet