Hi Vanessa:
I tried "Brizilliant" several years ago. I purchased the dry powder
since I have been mixing my own stains since the 1970's. I even called
the vendor as I was unsure about the identity of some of the
ingredients. The results on formalin fixed, paraffin embedded rodent
tissues were disappointing, a dull orange not bright red. You might have
better results with the pre-mixed stain. Perhaps they wills send you a
small sample to try on your material.
Geoff
On 8/30/2011 9:15 AM, Vanessa Orsini wrote:
Hi everyone,
I'm Vanessa and i'm quite new in the histology field so i need some help...
i'm having problems with a LacZ staining on mouse fresh frozen tissue. I
managed to get a nice staining also if I think it's a bit variable but I cannot
find a good counterstain!!
I tried the nuclear fast red but it's not stable in an aqua-based mounting
medium and if I do the acohol row before mounting with pertex the morphology of
the sections is damaged and the lacz staining is a bit washed away (a problem
if the Bgal expression is low)
I then tried with ematoxilin, the staining is stable but it's more difficult to
discriminate between the blue of the lacZ and the blue of ematoxilin.
I find out that the brazilin is a red ematoxilin so i decided to try this one
but once the powder arrived i didn't received any instruction for dilution with
it I couldn't find anything on the net.
Does someone have experience with brazilin?
or do you know some other red counterstaining that I could use?
Thanks a lot
Vanessa
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583
mcaul...@umdnj.edu
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