Hi, when I do Lac Z, I counterstain with nuclear fast red, rinse in water, then use one of the aqueous mounting medias that have replaced Crystal Mount. http://www.emsdiasum.com/microscopy/technical/datasheet/17985-12.aspx Place enough to cover the section, drain off the excess, completely air dry, and then you can permanently coverslip with resin. This is also the procedure I use for AEC and even Oil Red O. Crystal Mount forms a thin "plastic" protective film over the section.
Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 8901 S. Santa Fe, Suite G Oklahoma City, OK 73139 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com From: Geoff McAuliffe <mcaul...@umdnj.edu> To: histonet@lists.utsouthwestern.edu Sent: Tuesday, August 30, 2011 11:34 AM Subject: Re: [Histonet] brazilin Hi Vanessa: I tried "Brizilliant" several years ago. I purchased the dry powder since I have been mixing my own stains since the 1970's. I even called the vendor as I was unsure about the identity of some of the ingredients. The results on formalin fixed, paraffin embedded rodent tissues were disappointing, a dull orange not bright red. You might have better results with the pre-mixed stain. Perhaps they wills send you a small sample to try on your material. Geoff On 8/30/2011 9:15 AM, Vanessa Orsini wrote: > Hi everyone, > > I'm Vanessa and i'm quite new in the histology field so i need some help... > > i'm having problems with a LacZ staining on mouse fresh frozen tissue. I > managed to get a nice staining also if I think it's a bit variable but I > cannot find a good counterstain!! > I tried the nuclear fast red but it's not stable in an aqua-based mounting > medium and if I do the acohol row before mounting with pertex the morphology > of the sections is damaged and the lacz staining is a bit washed away (a > problem if the Bgal expression is low) > I then tried with ematoxilin, the staining is stable but it's more difficult > to discriminate between the blue of the lacZ and the blue of ematoxilin. > I find out that the brazilin is a red ematoxilin so i decided to try this one > but once the powder arrived i didn't received any instruction for dilution > with it I couldn't find anything on the net. > > Does someone have experience with brazilin? > or do you know some other red counterstaining that I could use? > > > Thanks a lot > > Vanessa > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcaul...@umdnj.edu ********************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet