Interesting....we see APP on frozen sections with 6E10, but not in paraffin sections after citrate HIER. We use the monoclonal from Covance, 1:12000 overnight at 4C.
Brett Brett M. Connolly, Ph.D. Imaging Research Fellow Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_conno...@merck.com T- 215-652-2501 F- 215-993-6803 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Hobbs, Carl Sent: Wednesday, October 26, 2011 2:52 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Seeking Beta-Amyloid not needing FA pretreatment Be careful: HIER may expose not only extracellular amyloid plaques but also intracellular APP ( or...is it intracellular A beta ???? ;-)) Eg: with 6E10.... it is directed against an epitope found in APP and also A beta: if you do Formic acid, only extracellular plaques are positive...after HIER, there is also intracellular positivity. In my experience. Some examples of this can be found here, in the image gallery : http://www.immunoportal.com/ Whichever way you go....good luck. Carl Carl Hobbs Histology Manager Wolfson CARD School of Biomedical Sciences Kings College London Guys Campus SE1 1UL Tel: 020 78486813 Fax: 020 78486816 020 78486813 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
