I agree with Liz, its too short for big skins. I always call skins "chewy" because thats what they will be if not processed enough. In my experiance with this, skins that are small 1-2mm punch biopsies and shaves have done ok in a 15 min run for each with 30 min each for the formalins. Simular to what Carol showed here. Ask the Pathologist exactly what do they mean they look funny? washed out? etc because it could be the stain. Have you changed to any new reagents recently? Kim
________________________________ From: Elizabeth Chlipala <l...@premierlab.com> To: 'Carol Bryant' <cb...@lexclin.com>; "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Sent: Friday, November 4, 2011 1:37 PM Subject: [Histonet] RE: processing program for biopsies In my personal opinion I think the processing cycle is too short. I know that skin biopsies are tiny but we process all skin samples on longer processing cycles. Granted I'm in research but we use 45 minutes to 1 hour per station for all of our skin samples and they cut just fine. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Carol Bryant Sent: Friday, November 04, 2011 10:51 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] processing program for biopsies Importance: High Would you experienced histotechs mind to look at this program and see if it would cause skin biopsies to be poorly processed? I think too much time in alcohols. The pathologists say they look bad. Thanks in advance for your help! Station Solution Time Temp P/V Mix ------- ---------------------- ----- ----- ----- ------ 1 Formalin 0:20 OFF P/V Slow 2 Formalin 0:20 OFF P/V Slow 3 70% Alcohol 0:15 OFF P/V Slow 4 80% Alcohol 0:15 OFF P/V Slow 5 95% Alcohol 0:15 OFF P/V Slow 6 95% Alcohol 0:25 OFF P/V Slow 7 100% Alcohol 0:25 OFF P/V Slow 8 100% Alcohol 0:25 OFF P/V Slow 9 Xylene 0:35 OFF P/V Slow 10 Xylene 0:35 OFF P/V Slow 11 Paraffin 0:15 63C. P/V Slow 12 Paraffin 0:15 63C. P/V Slow 13 Paraffin 0:15 63C. P/V Slow 14 Paraffin 0:15 63C. P/V Slow Carol Bryant, CT (ASCP) Cytology/Histology Manager Lexington Clinic Phone (859) 258-4082 Fax (859) 258-4081 cb...@lexclin.com NOTICE OF CONFIDENTIALITY This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations. If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet