Only 40 minutes in formalin is a problem. Increase the formalin time to several hours and this should suffice for small 2-3mm punch biopsies of skin.
For larger skin sections the alcohol, xylene and wax times will need to be tripled at least (as well as fixing the skins for longer (3-4 hours on the machine would be great)). Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Carol Bryant Sent: Saturday, 5 November 2011 3:51 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] processing program for biopsies Importance: High Would you experienced histotechs mind to look at this program and see if it would cause skin biopsies to be poorly processed? I think too much time in alcohols. The pathologists say they look bad. Thanks in advance for your help! Station Solution Time Temp P/V Mix ------- ---------------------- ----- ----- ----- ------ 1 Formalin 0:20 OFF P/V Slow 2 Formalin 0:20 OFF P/V Slow 3 70% Alcohol 0:15 OFF P/V Slow 4 80% Alcohol 0:15 OFF P/V Slow 5 95% Alcohol 0:15 OFF P/V Slow 6 95% Alcohol 0:25 OFF P/V Slow 7 100% Alcohol 0:25 OFF P/V Slow 8 100% Alcohol 0:25 OFF P/V Slow 9 Xylene 0:35 OFF P/V Slow 10 Xylene 0:35 OFF P/V Slow 11 Paraffin 0:15 63C. P/V Slow 12 Paraffin 0:15 63C. P/V Slow 13 Paraffin 0:15 63C. P/V Slow 14 Paraffin 0:15 63C. P/V Slow Carol Bryant, CT (ASCP) Cytology/Histology Manager Lexington Clinic Phone (859) 258-4082 Fax (859) 258-4081 cb...@lexclin.com NOTICE OF CONFIDENTIALITY This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations. If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
