Amber, Optimizing the antibody is the first step of validation. Running the new protocol against previously stained cases is the second step and shows you are getting results as good as, if not better, with the new vs the old.
Cindi Robinson HT(ASCP) Mercy Medical Center Dunes Medical Laboratories 350 W Anchor Dr Dakota Dunes SD 57049 phone-712-279-2768 robin...@mercyhealth.com >>> Amber McKenzie <amber.mcken...@gastrodocs.net> 11/16/2011 12:20 PM >>> Just wondering what y'alls opinion is on validation: I don't really understand why optimization isn't enough. At that point, the pathologist has said what he wanted the stain to look like, so why do 3-10 positive slides on the new instrument to compare to previous slides from another instrument? Thanks! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
