Usually now I only run an H&E control when I get a new lot of hematoxylin or eosin to make sure the stain is good. In other labs (especially if they are a strict GLP lab) I have run a control daily. I think it is just dependent on how strict your QA department is.
Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Corrie Vernick Sent: Wednesday, November 23, 2011 8:52 AM To: Histonet Lists Help Subject: [Histonet] standard operating procedure H&E Hi, I am a student and I am working on an assignment writing a mock SOP for the H & E staining procedure, both progressive and regressive. One heading of the SOP is control. I read the book and tried to google for a good answer as to what this part of the SOP entails. I know that controls are run to make sure that the slides are staining properly before new reagents are used on patient tissue. I am wondering if what I should be putting under the control heading of the SOP is that a control should be run after every stain or reagent change, or if there is something I am missing. Thank you, Corrinne Vernick Keiser University Orlando, FL U.S.A. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
