The method is found in Culling, Ed. 2., p.251.
Solutions
1) Alcian blue
Alcian blue 8G 1% aqueous 50 mL
Acetic acid 1% aqueous 50 mL
Filter, and add 10-20 mg thymol.
2) Phosphomolybdic acid, 1% aqueous
3) Chlorantine fast red 5B, 0.5% aqueous
Method
1) Sections to water.
2) Ehrlich's hematoxylin 10-15 mins.
3) Tap water until blue.
4) Alcian blue solution (1), 10 minutes.
5) Distilled water rinse, a few seconds.
6) Phosphomolybdic acid (2), 10 minutes.
7) Distilled water rinse.
8) Chlorantine fast red (3), 10-15 minutes.
9) Distilled water rinse.
10) Dehydrate, clear and mount in a resinous medium.
Expected results:
Mucin, mast cell granules, cartilage - blue
Nuclei - Purple-blue
Collagen and ossein - Cherry-red
Cytoplasm and muscle - pale yellow
Bryan Llewellyn
Ross, Catherine wrote:
All,
Greetings from across the pond!
One of our histology technicians is looking for a recipe for an alcian
blue/sirius red stain, otherwise known as "Alcian blue 8G with chlorantine
fast red 5B". It is not one I have used but I suggested tapping into the
wealth of knowledge on this forum - can anyone help, please?
Thank you in advance,
Catherine
Dr. Catherine L. Ross DVM MSc(VetPath) MRCVS
Covance Laboratories (UK) Ltd.
Otley Road,
Harrogate, N. Yorks.
HG3 1PY
T: +44 (0) 1423 848759
E: catherine.r...@covance.com<mailto:catherine.r...@covance.com>
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