Naveeda Arshad (where?) asks: >>Does any one has idea about how to make in house gram positive and negative control in your lab? What kind of tissue is suitable and and i need detail procedure for that?<<
Usually a section of a ruptured appendix (easy enough to get in a hospital histology lab) will provide an abundance of suitable bacteria. A better solution is not to do a tissue Gram stain at all. You want to see bacteria - you really can't identify them in tissue sections. A simple tissue Giemsa or Diff-Quik II stain is both sensitive and specific for seeing bacteria of all kinds. (The ruptured appendix will work well as a control.) Pathologists are much too ready to order a stain that's of very dubious clinical value, particularly since tissue Gram stains usually stain Gram negative organisms rather weakly. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
