But if you don't have a pathologist as compassionate as Dr Richmond and you are bound to do it. I would get microbiology to grow you a batch. Then plate/insert them into a section of lung. I prefer lung because it provides some background but not too much. This is just one way I've seen this done a long time ago. Good luck Kim Donadio
Sent from my iPhone On Dec 22, 2011, at 2:10 PM, Bob Richmond <rsrichm...@gmail.com> wrote: > Naveeda Arshad (where?) asks: >>Does any one has idea about how to > make in house gram positive and negative control in your lab? What > kind of tissue is suitable and and i need detail procedure for that?<< > > Usually a section of a ruptured appendix (easy enough to get in a > hospital histology lab) will provide an abundance of suitable > bacteria. > > A better solution is not to do a tissue Gram stain at all. You want to > see bacteria - you really can't identify them in tissue sections. A > simple tissue Giemsa or Diff-Quik II stain is both sensitive and > specific for seeing bacteria of all kinds. (The ruptured appendix will > work well as a control.) Pathologists are much too ready to order a > stain that's of very dubious clinical value, particularly since tissue > Gram stains usually stain Gram negative organisms rather weakly. > > Bob Richmond > Samurai Pathologist > Knoxville TN > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
