Naveeda, You can use a piece of fresh placenta or lung and just add the (+) & (-) gram bacteria (microbiology lab always have them in culture) to the tissues. Let the bugs infect the tissues for overnight @ room temperature or in a bacteria incubator (if you have one) @ 37c for overnight. I just add enough saline to the tissue and keep it moist. I chose placenta because it is easy to obtain (if there is a maternal ward, then plenty placenta after the delivery). Good Luck! Madeleine Supervisor-Pathology (IPOX & Histology) El Camino Hospital 2500 Grant Road Mountain View, CA 94040 madelein...@elcaminohospital.org
On Thu, Dec 22, 2011 at 10:54 AM, Madeleine Huey <madeleineh...@gmail.com> wrote: > Miha, > You need to lower down the cryostat at least -25 degree. You also need > to spray your block the very minute before the cutting (not just one > time spray on the OCT block). The thicker the section, the easier it > can be cut. > I also put my OCT blocks on the dry ice next to me, and transfer to > the cryostat when I am ready to cut. > Good Luck (I know it's not easy)! > Madeleine > Supervisor-Pathology (IPOX & Histology) > El Camino Hospital > > On Thu, Dec 22, 2011 at 10:00 AM, > <histonet-requ...@lists.utsouthwestern.edu> wrote: >> Send Histonet mailing list submissions to >> histonet@lists.utsouthwestern.edu >> >> To subscribe or unsubscribe via the World Wide Web, visit >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> or, via email, send a message with subject or body 'help' to >> histonet-requ...@lists.utsouthwestern.edu >> >> You can reach the person managing the list at >> histonet-ow...@lists.utsouthwestern.edu >> >> When replying, please edit your Subject line so it is more specific >> than "Re: Contents of Histonet digest..." >> >> >> Today's Topics: >> >> 1. out of office at noon today (marilyn.a.we...@kp.org) >> 2. TRAP Staining (Silvina Molinuevo) >> 3. Re: control block log (Richard Cartun) >> 4. Re: Histonet Digest, Vol 97, Issue 25 (naveeda arshad) >> 5. Re: Re: Histonet Digest, Vol 97, Issue 25 (Miha Tesar) >> 6. Position Update - SW Chicago Suburbs (Stephen G. Ruby) >> 7. equipment and supply donations for start up non profit >> histolab in DC (mad...@verizon.net) >> >> >> ---------------------------------------------------------------------- >> >> Message: 1 >> Date: Wed, 21 Dec 2011 10:01:33 -0800 >> From: marilyn.a.we...@kp.org >> Subject: [Histonet] out of office at noon today >> To: histonet@lists.utsouthwestern.edu >> Message-ID: >> <of5129baae.0f3a19fc-on8825796d.00630526-8825796d.00630...@kp.org> >> Content-Type: text/plain; charset=US-ASCII >> >> >> >> I will be out of the office starting 12/20/2011 and will not return until >> 12/27/2011. >> >> In my absence please ask for Mary . If this is urgent or you need to speak >> to me directly you can contact me on my cell phone number 858-472-4266. If >> it concerns a Mohs to be scheduled you can e-mail me or call on my cell. I >> will be in town for some of the time. >> Thank you. >> >> ------------------------------ >> >> Message: 2 >> Date: Wed, 21 Dec 2011 11:07:15 -0800 (PST) >> From: Silvina Molinuevo <silvinamolinu...@yahoo.com.ar> >> Subject: [Histonet] TRAP Staining >> To: "da...@uab.edu" <da...@uab.edu> >> Cc: "histonet@lists.utsouthwestern.edu" >> <histonet@lists.utsouthwestern.edu> >> Message-ID: >> <1324494435.42985.yahoomail...@web113610.mail.gq1.yahoo.com> >> Content-Type: text/plain; charset=iso-8859-1 >> >> Dear Dave, we use the next protocol with pretty good results. >> >> >> For diazotation reaction (Solution A): >> Dissolve 7mg Fast Garnet GBC base in 1ml of methyl or ethylenglygol. Add >> 1ml HCl 0.1N. Add drop by drop 2.07mg NO2Na. Keep T below 15ºC. >> >> Solution B:12.5mg/ml Naftol ASBI phosphate in distilled water >> >> For the enzymatic reaction: >> >> Disolve 75mg Sodium tartrate in 45 ml Citrate buffer pH 4.9. Pre-warm to 37ºC >> >> Add >> >> 2ml Solution A >> 0.5ml Solution B >> >> Incubate the slides about 1h at 37ºC. >> >> >> The most difficult thing is the diazotation reaction. >> >> You must have the stoichiometric relationship between Fast Garnet and Sodium >> nitrite. Sodium nitrite must be as pure as you can. You also have to retain >> the stoichiometry relation with chlorhidric acid to avoid reaction >> subproducts. Diazotation T must be not over 15ºC and you should use the >> diazotation product >> >> >> Best regards, silvina >> >> >> Dr María Silvina Molinuevo >> Grupo de Investigacion en >> Osteopatias y Metabolismo Mineral >> Departamento de Ciencias Biologicas >> Facultad de Ciencias Exactas >> Universidad Nacional de La Plata >> 47 y 115 >> (1900)La Plata >> Argentina >> www.biol.unlp.edu.ar/giomm >> e-mail: silvina.molinu...@bigfoot.com >> >> ------------------------------ >> >> Message: 3 >> Date: Wed, 21 Dec 2011 19:28:19 -0500 >> From: "Richard Cartun" <rcar...@harthosp.org> >> Subject: Re: [Histonet] control block log >> To: <stephen.cla...@hcahealthcare.com>, >> <Histonet@lists.utsouthwestern.edu> >> Message-ID: <4ef23353.7400.007...@harthosp.org> >> Content-Type: text/plain; charset=US-ASCII >> >> I use FileMaker Pro software to keep track of our IHC controls. I created a >> control tissue file that includes the following: Control #, Case #, Tissue >> site, Diagnosis, DOS, Time in formalin, Processing date, Number of blocks >> submitted, and then a "Comment" field for the immunoreactivity for each >> antibody tested. Every file can then be searched by anyone of the data >> fields. For example, if we need a control for Parvovirus we would type in >> "Parvovirus" in the "Diagnosis" or "Comment" fields and then every control >> tissue tested positive (or negative) for Parvovirus would be identified. >> >> Richard >> >> Richard W. Cartun, MS, PhD >> Director, Histology & Immunopathology >> Director, Biospecimen Collection Programs >> Assistant Director, Anatomic Pathology >> Hartford Hospital >> 80 Seymour Street >> Hartford, CT 06102 >> (860) 545-1596 Office >> (860) 545-2204 Fax >> >> >>>>> <stephen.cla...@hcahealthcare.com> 12/21/2011 10:25 AM >>> >> >> Hi. I was wondering if anyone would be willing to share a copy of their >> control block log. I'm creating a new one and want to see what others are >> doing, for reference. I'm just looking for a blank one. >> Thanks, >> >> Steve Clark >> Histology Supervisor >> Grand Strand Regional Medical Center >> 843-692-1486 Lab >> 843-692-1459 Desk >> stephen.cla...@hcahealthcare.com >> >> [cid:image001.gif@01C9DADA.65BB9E10] >> >> >> >> >> >> >> >> ------------------------------ >> >> Message: 4 >> Date: Wed, 21 Dec 2011 19:19:44 -0800 (PST) >> From: naveeda arshad <naveedafa...@yahoo.ca> >> Subject: [Histonet] Re: Histonet Digest, Vol 97, Issue 25 >> To: histonet@lists.utsouthwestern.edu >> Message-ID: >> <1324523984.99929.yahoomailclas...@web161704.mail.bf1.yahoo.com> >> Content-Type: text/plain; charset=iso-8859-1 >> >> Hi Does any one has idea about how to make in house gram Positive and >> negative control in your lab. What kind of tissue is suitable and and i need >> detail procedure for that.Thanks >> >> --- On Wed, 12/21/11, histonet-requ...@lists.utsouthwestern.edu >> <histonet-requ...@lists.utsouthwestern.edu> wrote: >> >> From: histonet-requ...@lists.utsouthwestern.edu >> <histonet-requ...@lists.utsouthwestern.edu> >> Subject: Histonet Digest, Vol 97, Issue 25 >> To: histonet@lists.utsouthwestern.edu >> Received: Wednesday, December 21, 2011, 10:17 PM >> >> Send Histonet mailing list submissions to >> histonet@lists.utsouthwestern.edu >> >> To subscribe or unsubscribe via the World Wide Web, visit >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> or, via email, send a message with subject or body 'help' to >> histonet-requ...@lists.utsouthwestern.edu >> >> You can reach the person managing the list at >> histonet-ow...@lists.utsouthwestern.edu >> >> When replying, please edit your Subject line so it is more specific >> than "Re: Contents of Histonet digest..." >> >> >> Today's Topics: >> >> 1. RE: Control Slides (Rene J Buesa) >> 2. Great Job Opportunity in San Fran Bay Area! (Heidi Hawthorne) >> >> >> ---------------------------------------------------------------------- >> >> Message: 1 >> Date: Wed, 21 Dec 2011 09:36:27 -0800 (PST) >> From: Rene J Buesa <rjbu...@yahoo.com> >> Subject: RE: [Histonet] Control Slides >> To: histonet@lists.utsouthwestern.edu, MargaretSherwood >> <msherw...@partners.org> >> Message-ID: >> <1324488987.20130.yahoomailclas...@web65716.mail.ac4.yahoo.com> >> Content-Type: text/plain; charset=iso-8859-1 >> >> Check with a colleague working in a hospital and ask for fresh tissue (for >> IHC) or (+) pathological cases. >> René J. >> >> --- On Wed, 12/21/11, Sherwood, Margaret <msherw...@partners.org> wrote: >> >> >> From: Sherwood, Margaret <msherw...@partners.org> >> Subject: RE: [Histonet] Control Slides >> To: "Rene J Buesa" <rjbu...@yahoo.com>, histonet@lists.utsouthwestern.edu >> Date: Wednesday, December 21, 2011, 12:15 PM >> >> >> >> >> That is ideal Rene, but we are a research lab and don't run the same tissue >> all the time. >> >> Peggy Sherwood >> Lab Associate, Photopathology >> Wellman Center for Photomedicine (EDR 214) >> Massachusetts General Hospital >> 50 Blossom Street >> Boston, MA 02114-2696 >> 617-724-4839 (voice mail) >> 617-726-6983 (lab) >> 617-726-1206 (fax) >> msherw...@partners.org >> >> >> >> >> From: Rene J Buesa [mailto:rjbu...@yahoo.com] >> Sent: Wednesday, December 21, 2011 12:05 PM >> To: histonet@lists.utsouthwestern.edu; Sherwood, Margaret >> Subject: Re: [Histonet] Control Slides >> >> >> >> >> >> >> >> I never bought a single (+) control slide. I prepared mine from the (+) >> cases we had. I think you should try this avenue as well. >> René J. >> >> --- On Wed, 12/21/11, Sherwood, Margaret <msherw...@partners.org> wrote: >> >> >> From: Sherwood, Margaret <msherw...@partners.org> >> Subject: Re: [Histonet] Control Slides >> To: histonet@lists.utsouthwestern.edu >> Date: Wednesday, December 21, 2011, 11:52 AM >> >> >> To all: >> >> I know this has come up before, but where do most people buy their (+) >> control >> slides for special stains? >> >> Thanks! >> Peggy >> >> Peggy Sherwood >> Lab Associate, Photopathology >> Wellman Center for Photomedicine (EDR 214) >> Massachusetts General Hospital >> 50 Blossom Street >> Boston, MA 02114-2696 >> 617-724-4839 (voice mail) >> 617-726-6983 (lab) >> 617-726-1206 (fax) >> msherw...@partners.org >> >> >> >> The information in this e-mail is intended only for the person to whom it is >> addressed. If you believe this e-mail was sent to you in error and the e-mail >> contains patient information, please contact the Partners Compliance >> HelpLine at >> http://www.partners.org/complianceline . If the e-mail was sent to you in >> error >> but does not contain patient information, please contact the sender and >> properly >> dispose of the e-mail. >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> ------------------------------ >> >> Message: 2 >> Date: Wed, 21 Dec 2011 09:41:24 -0800 >> From: Heidi Hawthorne <heidi.hawtho...@onassignment.com> >> Subject: [Histonet] Great Job Opportunity in San Fran Bay Area! >> To: "histonet@lists.utsouthwestern.edu" >> <histonet@lists.utsouthwestern.edu> >> Message-ID: >> >> <26c4a3b38503bc4cbbf4d986c3bc9b832290d1f...@oasslcexm01.oaifield.onasgn.com> >> >> Content-Type: text/plain; charset="us-ascii" >> >> Hello, >> >> We have an immediate need for a Histotechnician for our client in the East >> Bay of San Francisco. Approximately 3 month assignment, day/early morning >> shift available working in a hospital lab. Requirements: At least 6 months >> of paid experience in a hospital histology laboratory preparing and mounting >> pathological tissue specimens. >> >> Email your resume today for immediate consideration! >> >> Heidi Hawthorne >> Sr. Account Executive >> On Assignment, Inc. >> t: (510) 663-8622 >> c: (510) 435-7326 >> f: (866) 741-0805 >> heidi.hawtho...@onassignment.com<mailto:heidi.hawtho...@onassignment.com> >> www.onassignment.com<http://www.onassignment.com/> >> NASDAQ: ASGN >> >> >> People First. >> >> Find me on LinkedIn at: >> http://www.linkedin.com/pub/heidi-hawthorne/0/7b4/a39 >> >> >> >> ------------------------------ >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> End of Histonet Digest, Vol 97, Issue 25 >> **************************************** >> >> >> ------------------------------ >> >> Message: 5 >> Date: Wed, 21 Dec 2011 23:07:26 -0800 (PST) >> From: Miha Tesar <sauco...@yahoo.com> >> Subject: Re: [Histonet] Re: Histonet Digest, Vol 97, Issue 25 >> To: naveeda arshad <naveedafa...@yahoo.ca>, >> "histonet@lists.utsouthwestern.edu" >> <histonet@lists.utsouthwestern.edu> >> Message-ID: >> <1324537646.75201.yahoomail...@web45015.mail.sp1.yahoo.com> >> Content-Type: text/plain; charset=iso-8859-1 >> >> Haloha! >> Does any one has any idea about how to cut the fat tissue in cryostat >> (frozen section). Temperature of the chambre/specimen and the thickness of >> the slice. I use the cryo spray but does not help me much! >> THX Miha and Mary C. 2 all >> >> >> >> ________________________________ >> From: naveeda arshad <naveedafa...@yahoo.ca> >> To: histonet@lists.utsouthwestern.edu >> Sent: Thursday, December 22, 2011 4:19 AM >> Subject: [Histonet] Re: Histonet Digest, Vol 97, Issue 25 >> >> Hi Does make in house gram Positive and negative control in your lab. What >> kind of tissue is suitable and and i need detail procedure for that.Thanks >> >> --- On Wed, 12/21/11, histonet-requ...@lists.utsouthwestern.edu >> <histonet-requ...@lists.utsouthwestern.edu> wrote: >> >> From: histonet-requ...@lists.utsouthwestern.edu >> <histonet-requ...@lists.utsouthwestern.edu> >> Subject: Histonet Digest, Vol 97, Issue 25 >> To: histonet@lists.utsouthwestern.edu >> Received: Wednesday, December 21, 2011, 10:17 PM >> >> Send Histonet mailing list submissions to >> histonet@lists.utsouthwestern.edu >> >> To subscribe or unsubscribe via the World Wide Web, visit >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> or, via email, send a message with subject or body 'help' to >> histonet-requ...@lists.utsouthwestern.edu >> >> You can reach the person managing the list at >> histonet-ow...@lists.utsouthwestern.edu >> >> When replying, please edit your Subject line so it is more specific >> than "Re: Contents of Histonet digest..." >> >> >> Today's Topics: >> >> 1. RE: Control Slides (Rene J Buesa) >> 2. Great Job Opportunity in San Fran Bay Area! (Heidi Hawthorne) >> >> >> ---------------------------------------------------------------------- >> >> Message: 1 >> Date: Wed, 21 Dec 2011 09:36:27 -0800 (PST) >> From: Rene J Buesa <rjbu...@yahoo.com> >> Subject: RE: [Histonet] Control Slides >> To: histonet@lists.utsouthwestern.edu, MargaretSherwood >> <msherw...@partners.org> >> Message-ID: >> <1324488987.20130.yahoomailclas...@web65716.mail.ac4.yahoo.com> >> Content-Type: text/plain; charset=iso-8859-1 >> >> Check with a colleague working in a hospital and ask for fresh tissue (for >> IHC) or (+) pathological cases. >> René J. >> >> --- On Wed, 12/21/11, Sherwood, Margaret <msherw...@partners.org> wrote: >> >> >> From: Sherwood, Margaret <msherw...@partners.org> >> Subject: RE: [Histonet] Control Slides >> To: "Rene J Buesa" <rjbu...@yahoo.com>, histonet@lists.utsouthwestern.edu >> Date: Wednesday, December 21, 2011, 12:15 PM >> >> >> >> >> That is ideal Rene, but we are a research lab and don't run the same tissue >> all the time. >> >> Peggy Sherwood >> Lab Associate, Photopathology >> Wellman Center for Photomedicine (EDR 214) >> Massachusetts General Hospital >> 50 Blossom Street >> Boston, MA 02114-2696 >> 617-724-4839 (voice mail) >> 617-726-6983 (lab) >> 617-726-1206 (fax) >> msherw...@partners.org >> >> >> >> >> From: Rene J Buesa [mailto:rjbu...@yahoo.com] >> Sent: Wednesday, December 21, 2011 12:05 PM >> To: histonet@lists.utsouthwestern.edu; Sherwood, Margaret >> Subject: Re: [Histonet] Control Slides >> >> >> >> >> >> >> >> I never bought a single (+) control slide. I prepared mine from the (+) >> cases we had. I think you should try this avenue as well. >> René J. >> >> --- On Wed, 12/21/11, Sherwood, Margaret <msherw...@partners.org> wrote: >> >> >> From: Sherwood, Margaret <msherw...@partners.org> >> Subject: Re: [Histonet] Control Slides >> To: histonet@lists.utsouthwestern.edu >> Date: Wednesday, December 21, 2011, 11:52 AM >> >> >> To all: >> >> I know this has come up before, but where do most people buy their (+) >> control >> slides for special stains? >> >> Thanks! >> Peggy >> >> Peggy Sherwood >> Lab Associate, Photopathology >> Wellman Center for Photomedicine (EDR 214) >> Massachusetts General Hospital >> 50 Blossom Street >> Boston, MA 02114-2696 >> 617-724-4839 (voice mail) >> 617-726-6983 (lab) >> 617-726-1206 (fax) >> msherw...@partners.org >> >> >> >> The information in this e-mail is intended only for the person to whom it is >> addressed. If you believe this e-mail was sent to you in error and the e-mail >> contains patient information, please contact the Partners Compliance >> HelpLine at >> http://www.partners.org/complianceline . If the e-mail was sent to you in >> error >> but does not contain patient information, please contact the sender and >> properly >> dispose of the e-mail. >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> ------------------------------ >> >> Message: 2 >> Date: Wed, 21 Dec 2011 09:41:24 -0800 >> From: Heidi Hawthorne <heidi.hawtho...@onassignment.com> >> Subject: [Histonet] Great Job Opportunity in San Fran Bay Area! >> To: "histonet@lists.utsouthwestern.edu" >> <histonet@lists.utsouthwestern.edu> >> Message-ID: >> >> <26c4a3b38503bc4cbbf4d986c3bc9b832290d1f...@oasslcexm01.oaifield.onasgn.com> >> >> Content-Type: text/plain; charset="us-ascii" >> >> Hello, >> >> We have an immediate need for a Histotechnician for our client in the East >> Bay of San Francisco. Approximately 3 month assignment, day/early morning >> shift available working in a hospital lab. Requirements: At least 6 months >> of paid experience in a hospital histology laboratory preparing and mounting >> pathological tissue specimens. >> >> Email your resume today for immediate consideration! >> >> Heidi Hawthorne >> Sr. Account Executive >> On Assignment, Inc. >> t: (510) 663-8622 >> c: (510) 435-7326 >> f: (866) 741-0805 >> heidi.hawtho...@onassignment.com<mailto:heidi.hawtho...@onassignment.com> >> www.onassignment.com<http://www.onassignment.com/> >> NASDAQ: ASGN >> >> >> People First. >> >> Find me on LinkedIn at: >> http://www.linkedin.com/pub/heidi-hawthorne/0/7b4/a39 >> >> >> >> ------------------------------ >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> End of Histonet Digest, Vol 97, Issue 25 >> **************************************** >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> ------------------------------ >> >> Message: 6 >> Date: Thu, 22 Dec 2011 08:16:05 -0600 >> From: "Stephen G. Ruby" <sr...@4path.com> >> Subject: [Histonet] Position Update - SW Chicago Suburbs >> To: "histonet@lists.utsouthwestern.edu" >> <histonet@lists.utsouthwestern.edu> >> Message-ID: >> <E3C9B3B1471F0541AEF656B40F09DAB97147C4910A@4PATH-SBS01.4path.local> >> Content-Type: text/plain; charset="us-ascii" >> >> I had previously provided information on part time positions in the SW >> suburban location in Chicago. >> Currently, one of those positions has been upgraded to a full time position. >> >> If interested, please provide a CV and letter to me, and I will then forward. >> >> Thank you! >> >> Have a wonderful Holiday. >> >> Dr. Ruby >> >> >> ------------------------------ >> >> Message: 7 >> Date: Thu, 22 Dec 2011 09:44:20 -0600 (CST) >> From: mad...@verizon.net >> Subject: [Histonet] equipment and supply donations for start up non >> profit histolab in DC >> To: histonet@lists.utsouthwestern.edu >> Message-ID: <21850240.843147.1324568660991.JavaMail.root@vms170035> >> Content-Type: text/plain; charset="UTF-8" >> >> >> I am usually the one giving stuff away and I now find myself in need >> of a handout. If anyone in histoland has any items they can get rid of >> please email me back so we can work out some details. I can use >> anything in terms of supplies, expendables, equipment. I have a >> microtome and water bath past that I have an empty room. Many thanks! >> >> Nick(Rocky) Madary, HT/HTL(ASCP)QIHC >> >> >> ------------------------------ >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> End of Histonet Digest, Vol 97, Issue 26 >> **************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
