Adrienne, I have done this procedure before, I think. It sounds real familiar. I was taught it by some folks from Johns Hopkins a few years back for a nerve doctor here in Houston. I will look for the info and send it to you under a separate cover. If I don't have the procedure I may have the contact info for the folks at Johns Hopkins. The doctor in Houston has them come and train his new techs in the procedure. They are real nice and very helpful. Do you screen the biopsies for the fibers as well? I did, and did not like that aspect of the job, I felt that it was the doctor's responsibility to do that, not mine.
Toysha N. Mayer, MBA, HT (ASCP) Instructor Program in Histotechnology School of Health Professions MD Anderson Cancer Center (713) 563-3481 tnma...@mdanderson.org ------------------------------ Message: 5 Date: Tue, 3 Jan 2012 11:35:51 -0500 From: Adrienne Kavanagh <adrienneape...@hotmail.com> Subject: [Histonet] Nerve Fiber Density Testing To: <histonet@lists.utsouthwestern.edu> Message-ID: <bay167-w6fd3c32b174a280c4b68ec3...@phx.gbl> Content-Type: text/plain; charset="iso-8859-1" Hi All, I am setting up for a nerve fiber density test. The specifics of the procedure I have been provided with are vague. It basically involves taking a punch biopsy of skin, fixing in PLP, cryoprotecting, embedding in a sucrose solution to a frozen stage, cover with dry ice, and section using a sliding microtome at 50 microns. Then staining the free-floating sections with a PGP 9.5 antibody. If anyone is performing this testing, I would appreciate your response (you may contact me privately if you wish). Thanks! Adrienne ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet