I too am interested in setting up this test at my lab. I was curious as to why PLP was being used as a collection medium and if sections less than 50 um could be used. Other than fresh biopsies what other material would be suitable for validations?
Harpreet Singh, M.S. HTL (ASCP) In response to Message: 5 Date: Tue, 3 Jan 2012 11:35:51 -0500 From: Adrienne Kavanagh <adrienneape...@hotmail.com> Subject: [Histonet] Nerve Fiber Density Testing To: <histonet@lists.utsouthwestern.edu> Message-ID: <bay167-w6fd3c32b174a280c4b68ec3...@phx.gbl> Content-Type: text/plain; charset="iso-8859-1" Hi All, I am setting up for a nerve fiber density test. The specifics of the procedure I have been provided with are vague. It basically involves taking a punch biopsy of skin, fixing in PLP, cryoprotecting, embedding in a sucrose solution to a frozen stage, cover with dry ice, and section using a sliding microtome at 50 microns. Then staining the free-floating sections with a PGP 9.5 antibody. If anyone is performing this testing, I would appreciate your response (you may contact me privately if you wish). Thanks! Adrienne _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
