Also, using 100% alcohol on the cold plate induces cracking. Freeze spray does
too, although some people will never admit to using it. Both of these things
induce the rapid cooling effect.
Toysha N. Mayer, MBA, HT (ASCP)
Instructor
Program in Histotechnology
School of Health Professions
MD Anderson Cancer Center
(713) 563-3481
tnma...@mdanderson.org
------------------------------
------------------------------
Message: 8
Date: Wed, 4 Jan 2012 17:55:56 +0000 (UTC)
From: Pam Marcum <mucra...@comcast.net>
Subject: Re: [Histonet] Paraffin Blocks Cracking
To: Rene J Buesa <rjbu...@yahoo.com>
Cc: histonet@lists.utsouthwestern.edu
Message-ID:
<1488794099.223852.1325699756078.javamail.r...@sz0001a.westchester.pa.mail.comcast.net>
Content-Type: text/plain; charset=utf-8
I agree however; leaving blocks on the cold plate too long will also cause
cracking of blocks even at -2C.
----- Original Message -----
From: "Rene J Buesa" <rjbu...@yahoo.com>
To: histonet@lists.utsouthwestern.edu, "Brian foster" <brian1...@email.com>
Sent: Wednesday, January 4, 2012 11:50:13 AM
Subject: Re: [Histonet] Paraffin Blocks Cracking
Rapid cooling is the problem, but I do not think that -5??C can cause it. The
effect is more noticeable in large but thin blocks.
Ren?? J.
--- On Wed, 1/4/12, Brian foster <brian1...@email.com> wrote:
From: Brian foster <brian1...@email.com>
Subject: [Histonet] Paraffin Blocks Cracking
To: histonet@lists.utsouthwestern.edu
Date: Wednesday, January 4, 2012, 12:33 PM
Hello all,
I am studying for my HTL and have a question that has been a problem for me and
was wondering if anyione out there can help. "What cuases paraffin blocks to
crack ?" So far i have come up with it has to do the crystal formation during
cooling, and I have seen some Histonet articles that say the block cooling
plate at the embedding may be too cold ( less than -5 C). Can anyone confirm
that is the is the correct cause of the problem?
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End of Histonet Digest, Vol 98, Issue 4
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