We are currently starting up some IHC on frozen tissue sections.  After 
staining with different fluorescent antibodies, we end with applying DAPI 
w/Prolong gold and then coverslipping.  We would like to seal the coverslip so 
that we can keep the slides longer.

Any suggestions on where and how best to apply the nail polish for a permanent 
fix on the coverslips?

Thanks,

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