To: Jennifer K. Sipes Here's my suggestion;
1) Perfuse the mouse with 10% NFB (their legs & tail will move during perfusion, then harden if well perfused) 2) Remove intestine out & post-fix again with formalin for overnight 3) Fill a 10cc syringe with formalin & flush everything out 4) process & cut Good Luck! Madeleine Huey BS, HTL (ASCP) QIHC Supervisor - Pathology (IPOX & Histology) madelein...@elcaminohospital.org On Fri, Jan 27, 2012 at 8:10 AM, <histonet-requ...@lists.utsouthwestern.edu> wrote: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-requ...@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-ow...@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Re: Histonet Digest, Vol 98, Issue 36 (Jennifer Sipes) > 2. RE: Histonet Digest, Vol 98, Issue 35 (Reynolds,Donna M) > 3. Fwd: [Histonet] oct to paraffin (Emily Sours) > 4. RE: Re: Histonet Digest, Vol 98, Issue 36 (Truscott, Tom) > 5. RE: Interview Questions (Tony Henwood (SCHN)) > 6. RE: Oil red O versus Sudan 4 (Tony Henwood (SCHN)) > 7. Re: Re: Histonet Digest, Vol 98, Issue 36 (Kim Donadio) > 8. Re: Interview Questions (Joe Nocito) > 9. Precipitate (Matt Mincer) > 10. RE: Precipitate (Weems, Joyce) > 11. RE: [Histonet] (iskali...@bioacademy.gr) > 12. 2 New Perm Histology Opportunities (Matt Ward) > 13. RE: Precipitate (Morken, Timothy) > 14. Re: Precipitate (Akemi Allison) > 15. RE: Precipitate (Morken, Timothy) > 16. Gloves for coverslipping? (Tim Wheelock) > 17. Histotech opening Orange County California (Paula Lucas) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Thu, 26 Jan 2012 10:13:16 -0800 (PST) > From: Jennifer Sipes <jengirl1...@yahoo.com> > Subject: [Histonet] Re: Histonet Digest, Vol 98, Issue 36 > To: "histonet@lists.utsouthwestern.edu" > <histonet@lists.utsouthwestern.edu> > Message-ID: > <1327601596.45466.yahoomail...@web125402.mail.ne1.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Hi Histonetters! > > I have a question that I'm hoping you guys can help me with. I have a person > who brings me mouse intestinal tract with fecal matter still in it. She can > NOT remove the fecal matter before processing due to the fact that her lab is > studying the mucus membrane. She had asked the lab that was doing this how > they got such prestine sections while we are having a hard time with the > sections staying neat due to all the nicks caused by the fecal matter. Does > anyone have any suggestions as to how best to handle this?? It seems to be > getting worse the further into the study she goes. > > Thank you in advance for any help that you give!! It is truly appreciated! > > Jennifer K. Sipes, ALAT > Sr. Laboratory Technician > Johns Hopkins University > Ross 933 > 720 Rutland Avenue > Baltimore, MD 21205 > phone: 410-614-0131 > fax: 410-955-9677 > cell: 443-631-6361 > e-mail: jsip...@jhmi.edu > > ------------------------------ > > Message: 2 > Date: Thu, 26 Jan 2012 12:17:53 -0600 > From: "Reynolds,Donna M" <dreyn...@mdanderson.org> > Subject: [Histonet] RE: Histonet Digest, Vol 98, Issue 35 > To: "'histonet@lists.utsouthwestern.edu'" > <histonet@lists.utsouthwestern.edu> > Message-ID: > > <785bbf0c5f49ce41ba74460a43a08f023049632...@dcpwvmbxc0vs3.mdanderson.edu> > > Content-Type: text/plain; charset="us-ascii" > > > I can definitely see some of the pitfallls to having someone cut. But it > would certainly weed out anyone who didn't even know how to put a block in > the microtome. This is also what probation periods are for to see if this > person fits with your lab. > But have you ever heard of a secretary hired without a typing test or 9 key > if that was a heavy part of their job. Even with computers today this is > still done. > Donna > Houston, TX > > > Message: 3 > Date: Thu, 26 Jan 2012 02:59:49 +0000 (UTC) > From: koelli...@comcast.net > Subject: Re: [Histonet] Interview Questions > To: joelle weaver <joellewea...@hotmail.com> > Cc: Histonet <histonet@lists.utsouthwestern.edu> > Message-ID: > > <1983057611.84976.1327546789860.javamail.r...@sz0001a.emeryville.ca.mail.comcast.net> > > Content-Type: text/plain; charset=utf-8 > > This is certainly an interesting thread and I generally hate to get into > these ever but I still can't figure out one thing and never have over all > these years in pathology. What other endeavor in life and job seeking is an > on-the-spot demo that you can do something required at a job interview? Does > a lawyer have to go into a courtroom for 5 minutes and show he/she can say "I > object"? Does a sanitation worker have to go round the block once and show > he/she can empty 9 cans in 5 minutes? Does a doctor need to show he/she can > use a stethoscope? Does a bricklayer have to show he/she can lay 20 bricks in > 2 minutes? Or fail the interview? Does a med tech have to show they can stain > 6 tubes with CD4 and CD 8 and successfully put them on a flow cytometer? Does > an actuary have to show they can really add 100 4-digit numbers on a > calculator without a mistake? Does a grocery bagger boy /girl have to show > they can put x number of items in 3 bags? Does a Pathologist have to show > they know how to turn on a microscope and look through it? Does a peanut > counter have to show they can count peanuts? I just can't get into my mind > the necessity of someone having to cut to show they can cut? What other > profession does this at an interview? Now certainly you can come up with > scenarios where it might be important to find out. A brand new histotech > whose only cut 3 blocks in their life. A tech from the deepest, darkest > nether regions of the earth where you cannot check on their background. But a > tech whose has been working cutting the last 3 or 7 or 15 years and you've > verified with a previous company that is exactly what they did; how will them > cutting for 10 minutes further stratify them into yes or no categories. If 2 > potential techs cut and one finishes in 9 minutes and one in 10 minutes, is > that a true qualifier or disqualifier of what they can do cutting? There are > a myriad of things I'd love to know and always ask; personality, job > knowledge, wants, desires, needs, ambitions, etc, etc, etc. My blood pressure > skyrockets when I give blood because I HATE anyone sticking a needle in me. > But I have a really needed blood type. Should nervousness each time > disqualify me. This still boggles my mind about what is being accomplished > with cutting during an interview? > > > Ray > Seattle, WA > > > > > ------------------------------ > > Message: 3 > Date: Thu, 26 Jan 2012 14:24:46 -0500 > From: Emily Sours <talulahg...@gmail.com> > Subject: Fwd: [Histonet] oct to paraffin > To: histonet@lists.utsouthwestern.edu > Message-ID: > <CAP=xx1z1_ur98bcq5-e79ds_ntwt2d+rshca34o+radiibb...@mail.gmail.com> > Content-Type: text/plain; charset=UTF-8 > > I may have missed this but does one fix the embryo again in Carnoy's or > whatever after it has been washed (assuming it was fixed in the first place) > I would think the initial para fixation would be enough and (again after > washing in aqueous solution) dehydrating it up to xylene/toluene/non-aqeous > solution before the paraffin steps. > If one did fix again, would it be washed in 50% EtOH, 70% EtOH, before the > fix? > Or is this what the NBF washes are for? I have never used NBF, except when > I tried to make Serra's with it, and yeah, that's not a good idea. Always > use formalin, not NBF in Serra's or you'll get a lovely milky solution > that's not fit for fixing. > > Emily > > The whole point of this country is if you want to eat garbage, balloon up > to 600 pounds and die of a heart attack at 43, you can! You are free to do > so. To me, that’s beautiful. > --Ron Swanson > > > > On Wed, Jan 25, 2012 at 4:11 PM, Joseph Madary <pat...@gwumc.edu> wrote: > >> If you can spare some nbf the best thing to do would be to place the oct >> block right into NBF and use that as the "wash" and then move onto a fresh >> change of NBF. I would avoid straight water. NBF has enough water in it to >> rinse off the NBF, 1 or 2 changee for 15 minutes each is more than enough >> and a safe bet. >> >> Nick Madary, HT/HTL(ASCP)QIHC >> George Washington University >> Pathology Core Laboratory >> Ross Hall, Room 706 >> 23rd and I Street NW >> Washington D.C. 20037 >> 202.994.8196 >> pat...@gwumc.edu >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> > > > ------------------------------ > > Message: 4 > Date: Thu, 26 Jan 2012 21:31:01 +0000 > From: "Truscott, Tom" <ttrus...@vetmed.wsu.edu> > Subject: RE: [Histonet] Re: Histonet Digest, Vol 98, Issue 36 > To: Jennifer Sipes <jengirl1...@yahoo.com>, > "histonet@lists.utsouthwestern.edu" > <histonet@lists.utsouthwestern.edu> > Message-ID: > <9ef5279ebdfe6e4fb6605e8f183a002718833...@cvm76.vetmed.wsu.edu> > Content-Type: text/plain; charset="iso-8859-1" > > Hi Jennifer, I am by no means an expert on this, but have done a few mouse > guts. I think that trying to flush out the feces with formalin or saline soon > after necropsy, would help preserve the mucosa. The bacteria in the gut start > breaking down the mucosa soon after death. Perhaps the gut is thin enough to > fix rapidly enough to prevent damage to the mucosa without flushing. If that > is the case, then flushing out the feces after fixation might help the > quality of your slides. You may have to get permission to open up the gut to > flush out the feces. It may hinge on how the tissue needs to be trimmed and > oriented. Good luck, Tom Truscott > > -----Original Message----- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jennifer Sipes > Sent: Thursday, January 26, 2012 10:13 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Re: Histonet Digest, Vol 98, Issue 36 > > Hi Histonetters! > > I have a question that I'm hoping you guys can help me with. I have a person > who brings me mouse intestinal tract with fecal matter still in it. She can > NOT remove the fecal matter before processing due to the fact that her lab is > studying the mucus membrane. She had asked the lab that was doing this how > they got such prestine sections while we are having a hard time with the > sections staying neat due to all the nicks caused by the fecal matter. Does > anyone have any suggestions as to how best to handle this?? It seems to be > getting worse the further into the study she goes. > > Thank you in advance for any help that you give!! It is truly appreciated! > > Jennifer K. Sipes, ALAT > Sr. Laboratory Technician > Johns Hopkins University > Ross 933 > 720 Rutland Avenue > Baltimore, MD 21205 > phone: 410-614-0131 > fax: 410-955-9677 > cell: 443-631-6361 > e-mail: jsip...@jhmi.edu > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 5 > Date: Thu, 26 Jan 2012 21:39:59 +0000 > From: "Tony Henwood (SCHN)" <tony.henw...@health.nsw.gov.au> > Subject: RE: [Histonet] Interview Questions > To: "'Joe Nocito'" <jnoc...@satx.rr.com>, joelle weaver > <joellewea...@hotmail.com>, "trathbo...@somerset-healthcare.com" > <trathbo...@somerset-healthcare.com>, > "billodonn...@catholichealth.net" <billodonn...@catholichealth.net>, > "sbree...@nmda.nmsu.edu" <sbree...@nmda.nmsu.edu>, Histonet > <histonet@lists.utsouthwestern.edu> > Message-ID: <6d6bd1de8a5571489398b392a38a715760a16...@xmdb02.nch.kids> > Content-Type: text/plain; charset="us-ascii" > > Joe, > > I would never wear a denim miniskirt! > > Regards > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) > Laboratory Manager & Senior Scientist > Tel: 612 9845 3306 > Fax: 612 9845 3318 > the children's hospital at westmead > Cnr Hawkesbury Road and Hainsworth Street, Westmead > Locked Bag 4001, Westmead NSW 2145, AUSTRALIA > > -----Original Message----- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joe Nocito > Sent: Thursday, 26 January 2012 11:14 AM > To: joelle weaver; trathbo...@somerset-healthcare.com; > billodonn...@catholichealth.net; sbree...@nmda.nmsu.edu; Histonet > Subject: Re: [Histonet] Interview Questions > > I used to give a 10 question test on general histology. I also had the > expected answers written down and on my copy. Was accused once of being a > racist. What saved me was having the answers in front of me. The person > didn't get one answer correct. I had a couple of embedding questions, some > cutting, special stains, immunos and some QC questions. I gave the > interviewee the test while I was reviewing their resume. I would also see > what their facial expressions were too. I had one person tell me they didn't > do specials or immunos and didn't like embedding either. When I asked if they > liked filing blocks and slides, they really would rather have a lab aide do > it. This person didn't have to finish the test. Too make matters worse, she > wore a denim miniskirt to boot. Just my three cents > > Joe > ----- Original Message ----- > From: "joelle weaver" <joellewea...@hotmail.com> > To: <trathbo...@somerset-healthcare.com>; <billodonn...@catholichealth.net>; > <sbree...@nmda.nmsu.edu>; "Histonet" <histonet@lists.utsouthwestern.edu> > Sent: Wednesday, January 25, 2012 12:02 PM > Subject: RE: [Histonet] Interview Questions > > > > Love this! I always want to do demonstration during technical interviews, but > usually get "shot down" from managers and argued with in general, as in > people don't feel that they should have to "prove" they can do histology. > This perception, I never got, because I always saw it as in a job > interview-in what other situation are you more trying to "prove" or impress > with your knowledge, attitude, skills and experience? If you do bench work, > you can tell in just a few minutes of observation much more information than > you could get with quite a few questions. To be fair, I take into account > nervousness, being closely observed, and lack of familiarity with equipment > etc. I don't know, I think its fair if those are important skills to the > position/role. Was not sure if Sara's job was mostly technical though, so > thought I might keep it general. > > Joelle Weaver MAOM, (HTL) ASCP > > http://www.linkedin.com/in/joelleweaver > > > From: trathbo...@somerset-healthcare.com >> To: billodonn...@catholichealth.net; sbree...@nmda.nmsu.edu; >> histonet@lists.utsouthwestern.edu >> Date: Wed, 25 Jan 2012 17:47:01 +0000 >> Subject: RE: [Histonet] Interview Questions >> CC: >> >> If your replacement will be doing actual histology, will your >> institution permit the applicant to embed and cut? Can you sit down at >> a multi-head scope and review slides with them? >> What will the person be responsible for? Do they have experience with >> all of these tasks? What would they do in a crisis situation (you can >> make up one yourself that would be plausible). >> People who volunteer in their personal lives, may do the same at work. >> Ask how they juggle their schedule though, if there is a lot going on >> in their personal lives. Be careful with how you ask these questions >> though. Your HR department should be able to give you guidance in how to >> phrase things. >> Good luck. >> >> -----Original Message----- >> From: histonet-boun...@lists.utsouthwestern.edu >> [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of >> O'Donnell, Bill >> Sent: Wednesday, January 25, 2012 12:19 PM >> To: Breeden, Sara; histonet@lists.utsouthwestern.edu >> Subject: RE: [Histonet] Interview Questions >> >> It would seem that questions like "How do you feel about cannibalism?" >> might also be out but might be far more helpful; than "phone" questions. >> >> >> On the serious side, when I was much younger I hired a person who was >> able to answer all the right "histo" questions and so I hired him. He >> turned out to be a poser, who, shortly after I fired him showed up at >> a local university with a lab coat that listed him as "Dr." He had >> indeed worked in a histo lab, but as a lab assistant, and so the the >> understanding of what a histologist does was well rehearsed. (BTW, it >> topok me about two weeks to catch on, though the more experienced >> techs in the department figured it out almost right away) >> >> To be fair, it was during a time in hiring history when HR departments >> were not willing to give useful reference data and there were only a >> handful of questions they would even ask when checking. None of them >> were particularly useful or telling. For inistance, they would not ask >> if the person was an histo tech, but would simply ask, did he indeed >> work at your institution? >> >> The place where I worked required little or nothing for proof of >> experience. There was no background check either. >> >> Today, however, reference checking is a lot easier and more reliable. >> >> I guess my point here is that a good reference check needs to be done >> as well weeding them out by histo questions. I'm sure your HR folks >> will do a fine job of this. >> >> Also, once you have determined that they actually have the skills, or >> a realistic potential of gaining them, questions concerning dynamics >> of interaction are appropriate, though may lead to wrong impressions >> in the mind of the applicant. >> >> -----Original Message----- >> From: histonet-boun...@lists.utsouthwestern.edu >> [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of >> Breeden, Sara >> Sent: Wednesday, January 25, 2012 10:52 AM >> To: histonet@lists.utsouthwestern.edu >> Subject: [Histonet] Interview Questions >> >> So far, I am TOTALLY impressed and so grateful for your suggestions. >> And here's why... did I ever tell anyone out there what the FIRST >> question I was asked by the pathologist at my interview? It was..... >> (wait for it....) >> >> >> >> "How do you feel about personal phone calls?". Un-freakin' believable. >> I sure don't want someone to remember ME that way!!! >> >> >> >> Sally Breeden, HT(ASCP) >> >> New Mexico Department of Agriculture >> >> Veterinary Diagnostic Services >> >> 1101 Camino de Salud NE >> >> Albuquerque, NM 87102 >> >> 505-383-9278 (Histology Lab) >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> This electronic mail and any attached documents are intended solely >> for the named addressee(s) and contain confidential information. If >> you are not an addressee, or responsible for delivering this email to >> an addressee, you have received this email in error and are notified >> that reading, copying, or disclosing this email is prohibited. If you >> received this email in error, immediately reply to the sender and >> delete the message completely from your computer system. >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> CONFIDENTIALITY NOTICE >> This message and any included attachments are from Somerset Medical >> Center and are intended only for the addressee. The information >> contained in this message is confidential and may contain privileged, >> confidential, proprietary and/or trade secret information entitled to >> protection and/or exemption from disclosure under applicable law. >> Unauthorized forwarding, printing, copying, distribution, or use of >> such information is strictly prohibited and may be unlawful. If you >> are not the addressee, please promptly delete this message and notify >> the sender of the delivery error by e-mail or you may call Somerset >> Medical Center's computer Help Desk at 908-685-2200, ext. 4050. >> >> Be sure to visit Somerset Medical Center's Web site - >> www.somersetmedicalcenter.com - for the most up-to-date news, event >> listings, health information and more. >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ********************************************************************************* > This email and any files transmitted with it are confidential and intended > solely for the use of the individual or entity to whom they are addressed. If > you are not the intended recipient, please delete it and notify the sender. > > Views expressed in this message and any attachments are those of the > individual sender, and are not necessarily the views of The Children's > Hospital at Westmead > > This note also confirms that this email message has been virus scanned and > although no computer viruses were detected, The Childrens Hospital at > Westmead accepts no liability for any consequential damage resulting from > email containing computer viruses. > ********************************************************************************* > > > > ------------------------------ > > Message: 6 > Date: Thu, 26 Jan 2012 21:48:08 +0000 > From: "Tony Henwood (SCHN)" <tony.henw...@health.nsw.gov.au> > Subject: RE: [Histonet] Oil red O versus Sudan 4 > To: "'Candice Smoots'" <candice_cami...@yahoo.com>, Histonet > <histonet@lists.utsouthwestern.edu> > Message-ID: <6d6bd1de8a5571489398b392a38a715760a16...@xmdb02.nch.kids> > Content-Type: text/plain; charset="iso-8859-1" > > Candice, > > Remember freshly prepared Oil Red O Stock usually does not work. > Prepare your saturated Oil Red O Stock and leave it for a week or more before > using it to prepare a working solution. > > Regards > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) > Laboratory Manager & Senior Scientist > Tel: 612 9845 3306 > Fax: 612 9845 3318 > the children's hospital at westmead > Cnr Hawkesbury Road and Hainsworth Street, Westmead > Locked Bag 4001, Westmead NSW 2145, AUSTRALIA > > > -----Original Message----- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Candice Smoots > Sent: Thursday, 26 January 2012 3:21 AM > To: Histonet > Subject: [Histonet] Oil red O versus Sudan 4 > > Hi Histonetters > > > I am staining the plaques in aorta. I perfuse my animal with pbs before I > biospy the aorta. I then pin the aorta down onto a wax plate and then i > stain the inside of the aorta for my plaques. > > This seems to work well with the Sudan 4 but not so much with the Oil red o. > The person who was doing the sudan 4 is no longer here and we have plenty of > Oil red o. My thinking was that it should stain the same but I am not getting > results with the Oil red o. > > My question is what is the difference and what should I do differently with > the Oil red o? > > Thanks so much for your help! > > I remain yours truely, > > Candice Camille > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ********************************************************************************* > This email and any files transmitted with it are confidential and intended > solely for the use of the individual or entity to whom they are addressed. If > you are not the intended recipient, please delete it and notify the sender. > > Views expressed in this message and any attachments are those of the > individual sender, and are not necessarily the views of The Children's > Hospital at Westmead > > This note also confirms that this email message has been virus scanned and > although no computer viruses were detected, The Childrens Hospital at > Westmead accepts no liability for any consequential damage resulting from > email containing computer viruses. > ********************************************************************************* > > > > ------------------------------ > > Message: 7 > Date: Thu, 26 Jan 2012 16:48:41 -0500 > From: Kim Donadio <one_angel_sec...@yahoo.com> > Subject: Re: [Histonet] Re: Histonet Digest, Vol 98, Issue 36 > To: "Truscott, Tom" <ttrus...@vetmed.wsu.edu> > Cc: "histonet@lists.utsouthwestern.edu" > <histonet@lists.utsouthwestern.edu>, Jennifer Sipes > <jengirl1...@yahoo.com> > Message-ID: <a456638a-f783-4625-9aa3-01b475bfb...@yahoo.com> > Content-Type: text/plain; charset=us-ascii > > Yep! That's sounds reasonable. Maybe you could use a syringe and flush the > fecal material out with formalin? This way you shouldn't have to open it up. > Best of luck! > Kim :D > > Sent from my iPhone > > On Jan 26, 2012, at 4:31 PM, "Truscott, Tom" <ttrus...@vetmed.wsu.edu> wrote: > >> Hi Jennifer, I am by no means an expert on this, but have done a few mouse >> guts. I think that trying to flush out the feces with formalin or saline >> soon after necropsy, would help preserve the mucosa. The bacteria in the gut >> start breaking down the mucosa soon after death. Perhaps the gut is thin >> enough to fix rapidly enough to prevent damage to the mucosa without >> flushing. If that is the case, then flushing out the feces after fixation >> might help the quality of your slides. You may have to get permission to >> open up the gut to flush out the feces. It may hinge on how the tissue needs >> to be trimmed and oriented. Good luck, Tom Truscott >> >> -----Original Message----- >> From: histonet-boun...@lists.utsouthwestern.edu >> [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jennifer >> Sipes >> Sent: Thursday, January 26, 2012 10:13 AM >> To: histonet@lists.utsouthwestern.edu >> Subject: [Histonet] Re: Histonet Digest, Vol 98, Issue 36 >> >> Hi Histonetters! >> >> I have a question that I'm hoping you guys can help me with. I have a >> person who brings me mouse intestinal tract with fecal matter still in it. >> She can NOT remove the fecal matter before processing due to the fact that >> her lab is studying the mucus membrane. She had asked the lab that was doing >> this how they got such prestine sections while we are having a hard time >> with the sections staying neat due to all the nicks caused by the fecal >> matter. Does anyone have any suggestions as to how best to handle this?? >> It seems to be getting worse the further into the study she goes. >> >> Thank you in advance for any help that you give!! It is truly appreciated! >> >> Jennifer K. Sipes, ALAT >> Sr. Laboratory Technician >> Johns Hopkins University >> Ross 933 >> 720 Rutland Avenue >> Baltimore, MD 21205 >> phone: 410-614-0131 >> fax: 410-955-9677 >> cell: 443-631-6361 >> e-mail: jsip...@jhmi.edu >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 8 > Date: Thu, 26 Jan 2012 17:01:04 -0600 > From: "Joe Nocito" <jnoc...@satx.rr.com> > Subject: Re: [Histonet] Interview Questions > To: "Tony Henwood \(SCHN\)" <tony.henw...@health.nsw.gov.au>, "joelle > weaver" <joellewea...@hotmail.com>, > <trathbo...@somerset-healthcare.com>, > <billodonn...@catholichealth.net>, <sbree...@nmda.nmsu.edu>, > "Histonet" <histonet@lists.utsouthwestern.edu> > Message-ID: <E65ED4376C9E4DC59955A8E0204D49E4@JoePC> > Content-Type: text/plain; format=flowed; charset="iso-8859-1"; > reply-type=original > > I would appreciate that Tony > ----- Original Message ----- > From: "Tony Henwood (SCHN)" <tony.henw...@health.nsw.gov.au> > To: "'Joe Nocito'" <jnoc...@satx.rr.com>; "joelle weaver" > <joellewea...@hotmail.com>; <trathbo...@somerset-healthcare.com>; > <billodonn...@catholichealth.net>; <sbree...@nmda.nmsu.edu>; "Histonet" > <histonet@lists.utsouthwestern.edu> > Sent: Thursday, January 26, 2012 3:39 PM > Subject: RE: [Histonet] Interview Questions > > > Joe, > > I would never wear a denim miniskirt! > > Regards > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) > Laboratory Manager & Senior Scientist > Tel: 612 9845 3306 > Fax: 612 9845 3318 > the children's hospital at westmead > Cnr Hawkesbury Road and Hainsworth Street, Westmead > Locked Bag 4001, Westmead NSW 2145, AUSTRALIA > > -----Original Message----- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joe Nocito > Sent: Thursday, 26 January 2012 11:14 AM > To: joelle weaver; trathbo...@somerset-healthcare.com; > billodonn...@catholichealth.net; sbree...@nmda.nmsu.edu; Histonet > Subject: Re: [Histonet] Interview Questions > > I used to give a 10 question test on general histology. I also had the > expected answers written down and on my copy. Was accused once of being a > racist. What saved me was having the answers in front of me. The person > didn't get one answer correct. I had a couple of embedding questions, some > cutting, special stains, immunos and some QC questions. I gave the > interviewee the test while I was reviewing their resume. I would also see > what their facial expressions were too. I had one person tell me they didn't > do specials or immunos and didn't like embedding either. When I asked if > they liked filing blocks and slides, they really would rather have a lab > aide do it. This person didn't have to finish the test. Too make matters > worse, she wore a denim miniskirt to boot. Just my three cents > > Joe > ----- Original Message ----- > From: "joelle weaver" <joellewea...@hotmail.com> > To: <trathbo...@somerset-healthcare.com>; <billodonn...@catholichealth.net>; > <sbree...@nmda.nmsu.edu>; "Histonet" <histonet@lists.utsouthwestern.edu> > Sent: Wednesday, January 25, 2012 12:02 PM > Subject: RE: [Histonet] Interview Questions > > > > Love this! I always want to do demonstration during technical interviews, > but usually get "shot down" from managers and argued with in general, as in > people don't feel that they should have to "prove" they can do histology. > This perception, I never got, because I always saw it as in a job > interview-in what other situation are you more trying to "prove" or impress > with your knowledge, attitude, skills and experience? If you do bench work, > you can tell in just a few minutes of observation much more information than > you could get with quite a few questions. To be fair, I take into account > nervousness, being closely observed, and lack of familiarity with equipment > etc. I don't know, I think its fair if those are important skills to the > position/role. Was not sure if Sara's job was mostly technical though, so > thought I might keep it general. > > Joelle Weaver MAOM, (HTL) ASCP > > http://www.linkedin.com/in/joelleweaver > > > From: trathbo...@somerset-healthcare.com >> To: billodonn...@catholichealth.net; sbree...@nmda.nmsu.edu; >> histonet@lists.utsouthwestern.edu >> Date: Wed, 25 Jan 2012 17:47:01 +0000 >> Subject: RE: [Histonet] Interview Questions >> CC: >> >> If your replacement will be doing actual histology, will your >> institution permit the applicant to embed and cut? Can you sit down at >> a multi-head scope and review slides with them? >> What will the person be responsible for? Do they have experience with >> all of these tasks? What would they do in a crisis situation (you can >> make up one yourself that would be plausible). >> People who volunteer in their personal lives, may do the same at work. >> Ask how they juggle their schedule though, if there is a lot going on >> in their personal lives. Be careful with how you ask these questions >> though. Your HR department should be able to give you guidance in how to >> phrase things. >> Good luck. >> >> -----Original Message----- >> From: histonet-boun...@lists.utsouthwestern.edu >> [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of >> O'Donnell, Bill >> Sent: Wednesday, January 25, 2012 12:19 PM >> To: Breeden, Sara; histonet@lists.utsouthwestern.edu >> Subject: RE: [Histonet] Interview Questions >> >> It would seem that questions like "How do you feel about cannibalism?" >> might also be out but might be far more helpful; than "phone" questions. >> >> >> On the serious side, when I was much younger I hired a person who was >> able to answer all the right "histo" questions and so I hired him. He >> turned out to be a poser, who, shortly after I fired him showed up at >> a local university with a lab coat that listed him as "Dr." He had >> indeed worked in a histo lab, but as a lab assistant, and so the the >> understanding of what a histologist does was well rehearsed. (BTW, it >> topok me about two weeks to catch on, though the more experienced >> techs in the department figured it out almost right away) >> >> To be fair, it was during a time in hiring history when HR departments >> were not willing to give useful reference data and there were only a >> handful of questions they would even ask when checking. None of them >> were particularly useful or telling. For inistance, they would not ask >> if the person was an histo tech, but would simply ask, did he indeed >> work at your institution? >> >> The place where I worked required little or nothing for proof of >> experience. There was no background check either. >> >> Today, however, reference checking is a lot easier and more reliable. >> >> I guess my point here is that a good reference check needs to be done >> as well weeding them out by histo questions. I'm sure your HR folks >> will do a fine job of this. >> >> Also, once you have determined that they actually have the skills, or >> a realistic potential of gaining them, questions concerning dynamics >> of interaction are appropriate, though may lead to wrong impressions >> in the mind of the applicant. >> >> -----Original Message----- >> From: histonet-boun...@lists.utsouthwestern.edu >> [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of >> Breeden, Sara >> Sent: Wednesday, January 25, 2012 10:52 AM >> To: histonet@lists.utsouthwestern.edu >> Subject: [Histonet] Interview Questions >> >> So far, I am TOTALLY impressed and so grateful for your suggestions. >> And here's why... did I ever tell anyone out there what the FIRST >> question I was asked by the pathologist at my interview? It was..... >> (wait for it....) >> >> >> >> "How do you feel about personal phone calls?". Un-freakin' believable. >> I sure don't want someone to remember ME that way!!! >> >> >> >> Sally Breeden, HT(ASCP) >> >> New Mexico Department of Agriculture >> >> Veterinary Diagnostic Services >> >> 1101 Camino de Salud NE >> >> Albuquerque, NM 87102 >> >> 505-383-9278 (Histology Lab) >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> This electronic mail and any attached documents are intended solely >> for the named addressee(s) and contain confidential information. If >> you are not an addressee, or responsible for delivering this email to >> an addressee, you have received this email in error and are notified >> that reading, copying, or disclosing this email is prohibited. If you >> received this email in error, immediately reply to the sender and >> delete the message completely from your computer system. >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> CONFIDENTIALITY NOTICE >> This message and any included attachments are from Somerset Medical >> Center and are intended only for the addressee. The information >> contained in this message is confidential and may contain privileged, >> confidential, proprietary and/or trade secret information entitled to >> protection and/or exemption from disclosure under applicable law. >> Unauthorized forwarding, printing, copying, distribution, or use of >> such information is strictly prohibited and may be unlawful. If you >> are not the addressee, please promptly delete this message and notify >> the sender of the delivery error by e-mail or you may call Somerset >> Medical Center's computer Help Desk at 908-685-2200, ext. 4050. >> >> Be sure to visit Somerset Medical Center's Web site - >> www.somersetmedicalcenter.com - for the most up-to-date news, event >> listings, health information and more. >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ********************************************************************************* > This email and any files transmitted with it are confidential and intended > solely for the use of the individual or entity to whom they are addressed. > If you are not the intended recipient, please delete it and notify the > sender. > > Views expressed in this message and any attachments are those of the > individual sender, and are not necessarily the views of The Children's > Hospital at Westmead > > This note also confirms that this email message has been virus scanned and > although no computer viruses were detected, The Childrens Hospital at > Westmead accepts no liability for any consequential damage resulting from > email containing computer viruses. > ********************************************************************************* > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 9 > Date: Thu, 26 Jan 2012 19:57:19 -0600 > From: Matt Mincer <techon...@comcast.net> > Subject: [Histonet] Precipitate > To: histonet@lists.utsouthwestern.edu > Message-ID: <4f22047f.9010...@comcast.net> > Content-Type: text/plain; charset=ISO-8859-1; format=flowed > > Hey Histonet, > > We have a client who is having an odd problem with their processor. They > are getting "sandy" clogs in station 3. The original thought was that it > was formalin salts but the texture and color was wrong. Also, station 3 > is 70% which should be weak enough. One of the techs mentioned in > passing that the water quality in their town was really bad. I think > that the problem is that, like formalin, the alcohol is causing > dissolved minerals to be released from the tap water they use to mix > their 70%. Has anyone seen this before or am I chasing a harebrained > theory? Any thoughts would be greatly appreciated. > > Thanks > Matt > > -- > Matthew Mincer > Tech One Biomedical Services > 159 N Marion Street, PMB163 > Oak Park, IL 60301 > (708) 383-6040 X 10 > fax (708) 383-6045 > cell (708) 822-3738 > > > > > ------------------------------ > > Message: 10 > Date: Fri, 27 Jan 2012 00:35:29 -0500 > From: "Weems, Joyce" <jwe...@sjha.org> > Subject: RE: [Histonet] Precipitate > To: "m...@techonebiomedical.com" <m...@techonebiomedical.com>, > "histonet@lists.utsouthwestern.edu" > <histonet@lists.utsouthwestern.edu> > Message-ID: > <92ad9b20a6c38c4587a9febe3a30e1640846c1e...@chexcms10.one.ads.che.org> > Content-Type: text/plain; charset="us-ascii" > > Could they use DI water to test that theory? > > > Joyce Weems > Pathology Manager > Saint Joseph's Hospital > 5665 Peachtree Dunwoody Rd NE > Atlanta, GA 30342 > 678-843-7376 - Phone > 678-843-7831 - Fax > > > -----Original Message----- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Matt Mincer > Sent: Thursday, January 26, 2012 20:57 > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Precipitate > > Hey Histonet, > > We have a client who is having an odd problem with their processor. They are > getting "sandy" clogs in station 3. The original thought was that it was > formalin salts but the texture and color was wrong. Also, station 3 is 70% > which should be weak enough. One of the techs mentioned in passing that the > water quality in their town was really bad. I think that the problem is that, > like formalin, the alcohol is causing dissolved minerals to be released from > the tap water they use to mix their 70%. Has anyone seen this before or am I > chasing a harebrained theory? Any thoughts would be greatly appreciated. > > Thanks > Matt > > -- > Matthew Mincer > Tech One Biomedical Services > 159 N Marion Street, PMB163 > Oak Park, IL 60301 > (708) 383-6040 X 10 > fax (708) 383-6045 > cell (708) 822-3738 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Confidentiality Notice: > This e-mail, including any attachments is the > property of Catholic Health East and is intended > for the sole use of the intended recipient(s). > It may contain information that is privileged and > confidential. Any unauthorized review, use, > disclosure, or distribution is prohibited. If you are > not the intended recipient, please delete this message, and > reply to the sender regarding the error in a separate email. > > > > > > ------------------------------ > > Message: 11 > Date: Fri, 27 Jan 2012 09:22:00 +0200 > From: iskali...@bioacademy.gr > Subject: RE: [Histonet] > To: histonet@lists.utsouthwestern.edu > Cc: histonet@lists.utsouthwestern.edu > Message-ID: > <d8f45e6d02f6a3e333590b95b72f0c79.squir...@webmail.bioacademy.gr> > Content-Type: text/plain;charset=iso-8859-7 > > please remove my name from the list. > many thanks in advance, > I. Skaliora > > > > > ------------------------------ > > Message: 12 > Date: Fri, 27 Jan 2012 08:15:44 -0500 > From: Matt Ward <m...@personifysearch.com> > Subject: [Histonet] 2 New Perm Histology Opportunities > To: histonet@lists.utsouthwestern.edu > Message-ID: <1a817c77dcb05a27c3c4341e4a80c...@mail.gmail.com> > Content-Type: text/plain; charset=ISO-8859-1 > > Good Morning Histonet, > > > > Personify has had 2 new permanent Histology opportunities become available > in the Northern IL area. > > > > Please contact me directly at mw@personifysearch to learn more. > > > > *The Company:* > > Well-established provider of consumables and medical device accessories for > clinical histology and research laboratories. The facility works closely > with our UK, German and Australian facilities in the development, > manufacture and marketing of products including processing reagents, > storage and specimen transport devices, cytology accessories and safety > products. > > This is a globally focused business with significant sales and operations > in the US, Europe and Asia Pacific as well as a direct presence in over 100 > countries. > > *The Opportunities:* > > (1) QA Histologist: The company currently has an opening for a Quality > Assurance Histologist to be based in Richmond IL. > > (2) Histologist: The company currently has an opening for a Histologist > to be based in Richmond IL. > > Salary: Based on Experience > > Other: Full benefits - 401k program/matching > > > *Education and Experience Required:* > > - Experience with tissue grossing, tissue processing and embedding > - Experience with sectioning paraffin embedded tissue as well as frozen > tissue > - Experience performing routine stains (H and E) as well as special stains > - Experience with formulation and production of routine laboratory reagents > and solutions > - Experience performing and documenting routine laboratory procedures > - Familiarity with compliance requirements in the medical device industry > - Proficiency in basic computer skills and with software applications such > as Microsoft Office > > Have a great day! > > > > Regards, > > > > > > Matt Ward > > *Account Executive* > > *Personify* > > 5020 Weston Parkway Suite 315 > > Cary NC 27513 > > (Tel) 800.875.6188 direct ext 103 > > (Fax) 919.460.0642 > > www.personifysearch.com > > > ------------------------------ > > Message: 13 > Date: Fri, 27 Jan 2012 06:23:50 -0800 > From: "Morken, Timothy" <timothy.mor...@ucsfmedctr.org> > Subject: RE: [Histonet] Precipitate > To: "'m...@techonebiomedical.com'" <m...@techonebiomedical.com>, > "histonet@lists.utsouthwestern.edu" > <histonet@lists.utsouthwestern.edu> > Message-ID: > > <8d7c2d242dbd45498006b21122072bf89f5ee...@mcinfrwem003.ucsfmedicalcenter.org> > > Content-Type: text/plain; charset=us-ascii > > Matt, we have seen that in one of our VIP5's and we do weekly hot-water rinse > of the first 4 stations. It is the oldest processor, at about 7 years. That > processor had frequent pump-in pump-out errors randomly in the first 3 > stations. Finally the service tech decided to "get to the bottom of it" and > he found the steel tubing clogged with "sand." It took a whole day to clean > it all out. It did not look like formalin salts, but did look like the kind > of deposits that you see with hard water in pipes. > > Since then, no problems at all. > > Tim Morken > Supervisor, Histology, IPOX > UCSF Medical Center > San Francisco, CA, USA > > -----Original Message----- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Matt Mincer > Sent: Thursday, January 26, 2012 5:57 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Precipitate > > Hey Histonet, > > We have a client who is having an odd problem with their processor. They are > getting "sandy" clogs in station 3. The original thought was that it was > formalin salts but the texture and color was wrong. Also, station 3 is 70% > which should be weak enough. One of the techs mentioned in passing that the > water quality in their town was really bad. I think that the problem is that, > like formalin, the alcohol is causing dissolved minerals to be released from > the tap water they use to mix their 70%. Has anyone seen this before or am I > chasing a harebrained theory? Any thoughts would be greatly appreciated. > > Thanks > Matt > > -- > Matthew Mincer > Tech One Biomedical Services > 159 N Marion Street, PMB163 > Oak Park, IL 60301 > (708) 383-6040 X 10 > fax (708) 383-6045 > cell (708) 822-3738 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 14 > Date: Fri, 27 Jan 2012 06:39:18 -0800 > From: Akemi Allison <akemiat3...@yahoo.com> > Subject: Re: [Histonet] Precipitate > To: "Morken, Timothy" <timothy.mor...@ucsfmedctr.org> > Cc: "histonet@lists.utsouthwestern.edu" > <histonet@lists.utsouthwestern.edu>, "'m...@techonebiomedical.com'" > <m...@techonebiomedical.com> > Message-ID: <44f7d4b6-ddbf-4e82-8c42-060a4583e...@yahoo.com> > Content-Type: text/plain; charset=US-ASCII; delsp=yes; > format=flowed > > I agree with Tim, but I am curious why tap water is being used to > make the 70% alcohol instead of DI water. Tap water has been known > to have all kinds of contaminates. > > > Akemi Allison BS, HT (ASCP) HTL > Director > Phoenix Lab Consulting > Tele: 408.335.9994 > E-Mail: akemiat3...@yahoo.com > > On Jan 27, 2012, at 6:23 AM, Morken, Timothy wrote: > >> Matt, we have seen that in one of our VIP5's and we do weekly hot- >> water rinse of the first 4 stations. It is the oldest processor, >> at about 7 years. That processor had frequent pump-in pump-out >> errors randomly in the first 3 stations. Finally the service tech >> decided to "get to the bottom of it" and he found the steel tubing >> clogged with "sand." It took a whole day to clean it all out. It >> did not look like formalin salts, but did look like the kind of >> deposits that you see with hard water in pipes. >> >> Since then, no problems at all. >> >> Tim Morken >> Supervisor, Histology, IPOX >> UCSF Medical Center >> San Francisco, CA, USA >> >> -----Original Message----- >> From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet- >> boun...@lists.utsouthwestern.edu] On Behalf Of Matt Mincer >> Sent: Thursday, January 26, 2012 5:57 PM >> To: histonet@lists.utsouthwestern.edu >> Subject: [Histonet] Precipitate >> >> Hey Histonet, >> >> We have a client who is having an odd problem with their processor. >> They are getting "sandy" clogs in station 3. The original thought >> was that it was formalin salts but the texture and color was wrong. >> Also, station 3 is 70% which should be weak enough. One of the >> techs mentioned in passing that the water quality in their town was >> really bad. I think that the problem is that, like formalin, the >> alcohol is causing dissolved minerals to be released from the tap >> water they use to mix their 70%. Has anyone seen this before or am >> I chasing a harebrained theory? Any thoughts would be greatly >> appreciated. >> >> Thanks >> Matt >> >> -- >> Matthew Mincer >> Tech One Biomedical Services >> 159 N Marion Street, PMB163 >> Oak Park, IL 60301 >> (708) 383-6040 X 10 >> fax (708) 383-6045 >> cell (708) 822-3738 >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 15 > Date: Fri, 27 Jan 2012 07:17:33 -0800 > From: "Morken, Timothy" <timothy.mor...@ucsfmedctr.org> > Subject: RE: [Histonet] Precipitate > To: "'Akemi Allison'" <akemiat3...@yahoo.com> > Cc: "histonet@lists.utsouthwestern.edu" > <histonet@lists.utsouthwestern.edu>, "'m...@techonebiomedical.com'" > <m...@techonebiomedical.com> > Message-ID: > > <8d7c2d242dbd45498006b21122072bf89f5ee...@mcinfrwem003.ucsfmedicalcenter.org> > > Content-Type: text/plain; charset=us-ascii > > I was/am wondering because we do use Di water for our 80% (our lowest ETOH). > But it that processor was used in a different lab for several years so maybe > it got started there. > > None of our other 3 processors had the problem. > > Tim Morken > Supervisor, Histology, IPOX > UCSF Medical Center > San Francisco, CA, USA > From: Akemi Allison [mailto:akemiat3...@yahoo.com] > Sent: Friday, January 27, 2012 6:39 AM > To: Morken, Timothy > Cc: 'm...@techonebiomedical.com'; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Precipitate > > I agree with Tim, but I am curious why tap water is being used to make the > 70% alcohol instead of DI water. Tap water has been known to have all kinds > of contaminates. > > > Akemi Allison BS, HT (ASCP) HTL > Director > Phoenix Lab Consulting > Tele: 408.335.9994 > E-Mail: akemiat3...@yahoo.com<mailto:akemiat3...@yahoo.com> > > On Jan 27, 2012, at 6:23 AM, Morken, Timothy wrote: > > > Matt, we have seen that in one of our VIP5's and we do weekly hot-water rinse > of the first 4 stations. It is the oldest processor, at about 7 years. That > processor had frequent pump-in pump-out errors randomly in the first 3 > stations. Finally the service tech decided to "get to the bottom of it" and > he found the steel tubing clogged with "sand." It took a whole day to clean > it all out. It did not look like formalin salts, but did look like the kind > of deposits that you see with hard water in pipes. > > Since then, no problems at all. > > Tim Morken > Supervisor, Histology, IPOX > UCSF Medical Center > San Francisco, CA, USA > > -----Original Message----- > From: > histonet-boun...@lists.utsouthwestern.edu<mailto:histonet-boun...@lists.utsouthwestern.edu> > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Matt Mincer > Sent: Thursday, January 26, 2012 5:57 PM > To: > histonet@lists.utsouthwestern.edu<mailto:histonet@lists.utsouthwestern.edu> > Subject: [Histonet] Precipitate > > Hey Histonet, > > We have a client who is having an odd problem with their processor. They are > getting "sandy" clogs in station 3. The original thought was that it was > formalin salts but the texture and color was wrong. Also, station 3 is 70% > which should be weak enough. One of the techs mentioned in passing that the > water quality in their town was really bad. I think that the problem is that, > like formalin, the alcohol is causing dissolved minerals to be released from > the tap water they use to mix their 70%. Has anyone seen this before or am I > chasing a harebrained theory? Any thoughts would be greatly appreciated. > > Thanks > Matt > > -- > Matthew Mincer > Tech One Biomedical Services > 159 N Marion Street, PMB163 > Oak Park, IL 60301 > (708) 383-6040 X 10 > fax (708) 383-6045 > cell (708) 822-3738 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu<mailto:Histonet@lists.utsouthwestern.edu> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu<mailto:Histonet@lists.utsouthwestern.edu> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 16 > Date: Fri, 27 Jan 2012 10:48:05 -0500 > From: Tim Wheelock <twheel...@mclean.harvard.edu> > Subject: [Histonet] Gloves for coverslipping? > To: histonet@lists.utsouthwestern.edu > Message-ID: <4f22c735.1060...@mclean.harvard.edu> > Content-Type: text/plain; charset=ISO-8859-1; format=flowed > > Hi Everybody: > > Does anyone know of a xylene-resistant glove that can be used for > coverslipping, and that allows the dexterity necessary to coverslip? > > Thanks, > > Tim Wheelock > Harvard Brain Bank > McLean Hospital > Belmont, MA > > > > > ------------------------------ > > Message: 17 > Date: Fri, 27 Jan 2012 08:10:27 -0800 > From: "Paula Lucas" <plu...@biopath.org> > Subject: [Histonet] Histotech opening Orange County California > To: <histonet@lists.utsouthwestern.edu> > Message-ID: <03F37C569B91460393E1E27893BCD837@biopath.local> > Content-Type: text/plain; charset="us-ascii" > > We have a part time histotech position available working from 5 am on > Tuesday through Saturdays. We are located in Fountain Valley, California. > > Please provide me a summary of your histotech experience and please send me > your resume. > > Thank you, > > Paula Lucas > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 98, Issue 37 > **************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
