We have been performing C4d IHC on FFPE kidney for a couple of years. We receive only fixed samples. Yesterday my doctors came with this idea of IF - I still have to ask for the special reasons. But I have the suspicion, that they are not aware of the fact, that IF on frozen unfixed tissue is the usual way found in literature.
There are some articles that deal with comparison of IF(frozen) and IHC(ffpe). The results are usually an equal outcome. IF(frozen) shows additional staining in glomeruli. Perhaps someone told them, that IF is the golden standard and recommended. Gudrun -----Ursprüngliche Nachricht----- Von: Richard Cartun [mailto:rcar...@harthosp.org] Gesendet: Mittwoch, 01. Februar 2012 21:21 An: gu.l...@gmx.at Betreff: Re: [Histonet] C4d IF on FFPE kidney Hi Gudrun: Is there reason why you want to use IF and not immunoperoxidase? Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> "Gudrun Lang" <gu.l...@gmx.at> 2/1/2012 3:17 PM >>> Hi! Can someone provide me a immunofluorescence protocol for C4d on formalin fixed human paraffin sections? thanks in advance Gudrun Lang _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet