Hi Gudren We have been doing both IF and IHC for C4d for quite a while. While we have found that the IF staining is superior to the IHC we are not unhappy with the IHC results. Our IHC results have improved since getting a Ventana Ultra which has allowed better control of the staining. regards Tony
Tony Reilly B.App.Sc. , M.Sc. Chief Scientist, Anatomical Pathology Pathology Queensland-PA Laboratory _________________________________________________ Clinical and Statewide Services Division| QueenslandHealth Level 1, Building 15,Princess Alexandra Hospital Ipswich Road,WOOLLOONGABBA Qld4102 Ph: 07 3176 2412 Mob: 0402 139411 Fax: 07 3176 2930 Email: tony_rei...@health.qld.gov.au Web: www.health.qld.gov.au/qhcss/ >>> "Gudrun Lang" <gu.l...@gmx.at> 2/3/2012 12:31 am >>> We have been performing C4d IHC on FFPE kidney for a couple of years. We receive only fixed samples. Yesterday my doctors came with this idea of IF - I still have to ask for the special reasons. But I have the suspicion, that they are not aware of the fact, that IF on frozen unfixed tissue is the usual way found in literature. There are some articles that deal with comparison of IF(frozen) and IHC(ffpe). The results are usually an equal outcome. IF(frozen) shows additional staining in glomeruli. Perhaps someone told them, that IF is the golden standard and recommended. Gudrun -----Ursprüngliche Nachricht----- Von: Richard Cartun [mailto:rcar...@harthosp.org] Gesendet: Mittwoch, 01. Februar 2012 21:21 An: gu.l...@gmx.at Betreff: Re: [Histonet] C4d IF on FFPE kidney Hi Gudrun: Is there reason why you want to use IF and not immunoperoxidase? Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> "Gudrun Lang" <gu.l...@gmx.at> 2/1/2012 3:17 PM >>> Hi! Can someone provide me a immunofluorescence protocol for C4d on formalin fixed human paraffin sections? thanks in advance Gudrun Lang _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ******************************************************************************** This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. ********************************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet