The shiny side (block side) goes on the water so when you do your block to slide comparison the slide WILL match the block. We routinely perform a percentage of slide/block matches for quality control. Some organizations perform 100% slide/block match as a final QC check. Jackie O'
-----Original Message----- From: Eric Hoy <eric....@utsouthwestern.edu> To: Histonet <histonet@lists.utsouthwestern.edu> Sent: Tue, Feb 28, 2012 7:36 pm Subject: Re: [Histonet] Shiny side of a paraffin section All of the cells would be face down when you looked at them! (It's already been a long week!) Eric Hoy =============================================== ric S. Hoy, Ph.D., SI(ASCP) linical Associate Professor epartment of Medical Laboratory Sciences he University of Texas Southwestern Medical Center allas, Texas mail: eric....@utsouthwestern.edu ============================================== n 2/28/12 5:56 PM, "Lucie Guernsey" <lguern...@ucsd.edu> wrote: > As all of us who cut paraffin know, the underside of each section as it comes off the blade is shiny. I've always accepted it as a fact that the shiny side always goes down on the water bath, but I've begun to wonder why. Is there a specific reason why we're all taught to put the shiny side down? What would the difference be between a 'properly' collected section and a rebelliously collected shiny-side up section? Does it even matter? Thanks! Lucie Lucie Guernsey UC San Diego lguern...@ucsd.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ istonet mailing list isto...@lists.utsouthwestern.edu ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
