My mentor, Nick Roman, told me that sections adhere to the slide better if they go on shiny side down. Brenda Disbrey's HISTOLOGICAL LABORATORY METHODS says that laying the sections on the water bath or water droplet shiny side down makes it easier to remove creases. Benno Romeiss' MIKROSKOPISCHE TECHNIK and Manfred Gabe's TECHNIQUES HISTOLOGIQUES say that section should be mounted shiny side down without giving a reason. Most other authors do not even mention this matter.
Allen A. Smith Professor of Anatomy Barry University School of Podiatric Medicine Miami Shores, Florida -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lucie Guernsey Sent: Tuesday, February 28, 2012 6:56 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Shiny side of a paraffin section As all of us who cut paraffin know, the underside of each section as it comes off the blade is shiny. I've always accepted it as a fact that the shiny side always goes down on the water bath, but I've begun to wonder why. Is there a specific reason why we're all taught to put the shiny side down? What would the difference be between a 'properly' collected section and a rebelliously collected shiny-side up section? Does it even matter? Thanks! Lucie Lucie Guernsey UC San Diego lguern...@ucsd.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
