Why do you want the pH so low? Citrate buffers are most effective in the range of pH 3.0-6.0.
I would check with the company and make sure that the pH is supposed to be 2.0, and ask them for a formula to achieve such a solution. It probably won't have much buffering capacity at pH 2.0. There is a Java application that will calculate formulae for citrate buffers, but it will point out that the buffer should be used close to one of the three pK's of citric acid: http://www.egr.msu.edu/scb-group/tools/citric/cit.htm Good luck! Eric Hoy =============================================== Eric S. Hoy, Ph.D., SI(ASCP) Clinical Associate Professor Department of Medical Laboratory Sciences The University of Texas Southwestern Medical Center Dallas, Texas Email: eric....@utsouthwestern.edu =============================================== On 4/17/12 11:25 AM, "Neil Macintyre" <neil.macint...@ed.ac.uk> wrote: > Hi > Can anyone help me with a formula for 0.01M citrate buffer pH2.0 for antigen > retrieval as this pH has been recommended by the company supplying AM3K > macrophage antibody and I can't find this in any of the usual IHC buffer > protocols. > Thanks > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
