Neil:
I haven't made stock solution recently, but I believe the starting pH of
solution prior to adding NaOH is less than 2, so this should work.
Citrate pH 3.0 use at 10 mM
For a 10 x stock solution [100 mM]
10.5 g citric acid monohydrate [MW 210.14] in 370 ml water
adjust the pH to 3.0 with concentrated NaOH
add 2.5 ml Tween 20
adjust final volume to 500 ml
Tresa
Tresa Goins
Montana Veterinary Diagnostic Lab
Bozeman, Montana 59718
406-994-6353
-----Original Message-----
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Neil Macintyre
Sent: Tuesday, April 17, 2012 10:26 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Citrate Buffer pH2.0
Hi
Can anyone help me with a formula for 0.01M citrate buffer pH2.0 for antigen
retrieval as this pH has been recommended by the company supplying AM3K
macrophage antibody and I can't find this in any of the usual IHC buffer
protocols.
Thanks
Neil MacIntyre CSci FIBMS
Laboratory Manager
Veterinary Pathology Unit
The Royal(Dick)School of Veterinary Studies The University Of Edinburgh Easter
Bush Campus Midlothian
EH25 9RG
0131 650 6403/8802
The University of Edinburgh is a charitable body, registered in Scotland, with
registration number SC005336.
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