Hi Histonetters,
 
Does anybody has experience in making your own HP control tissue? I am tired of 
using positive biopsies of patients since they are always almost finished.  I 
have heard of a procedure on making HP controls but I am not exactly sure how 
it is done.
 
I have tried the following: I went to the microbiology department and asked for 
some freshly grown HP bacteria. We scraped them of the petridishes and put them 
in formalin. I had them fixed for 24 hours and then I injected them into some 
already fixed lung tissue and processed it as normal. But if I do a HP stain on 
that tissue, I don't see any bacteria. Probably they rinse off during the 
process. 
 
Can anyone tell me how to do it? 
 
Thanks in advance,
 
Willem Hoekert
OLVG 
The Netherlands
 

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