You should not have to use retreival. Retrieval either by heat/solution or enzyme digestion is usually needed when the antigen/antibody site has been blocked/masked by the fixation process. Kim D
________________________________ From: Daniela Bodemer <daniela.bode...@mcri.edu.au> To: histonet@lists.utsouthwestern.edu Sent: Wednesday, July 4, 2012 7:02 AM Subject: [Histonet] Frozens and antigen retrieval Hi all, A question from my student: antigen retrieval when using frozens for immunofluorescence -yes or no? The protocol suggested by the antibody company lists the retrieval as an option. I am used to do retrieval on paraffin sections, but not on cryo sections. Hit me with your opinions on this :-) Thanks in advance, Daniela Sent from my iPad ______________________________________________________________________ This email has been scanned by the Symantec Email Security.cloud service. For more information please visit http://www.symanteccloud.com ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
