Hi Folks...I am hoping you all give me a little help. Our Pathologists are complaining about our Eosin on the H&E's being weak. The funny thing is, is that it can go one
day to the next...one day it looks great...the next it is weak!! I have already done some experimenting with...1) time tissue spends in Eosin.... 2) making sure that the alcohols after Eosin are the proper concentrations....3) reducing the time that the tissues spends in the alcohols atfer Eosin... I have even gone as far as 4) increasing the rinse time in water after the decolorizing and bluing steps. 5) I have checked the pH of the water as well. Any help and suggestions would greatly appreciated!! Thanks Gang!! Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish Medical Center 951 N. Washington Ave. Titusville, Florida 32976 Phone:(321) 268-6333 ext. 7506 Fax: (321) 268-6149 valerie.han...@parrishmed.com * ============================================================= "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" =============================================================_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet