Do you recycle your alcohols? If you do and you put the recycled alcohols at the end (running down to xylene) it can GREATLY decolorize the eosin in the H&E stain. Also, make sure you have a good rinse after the blueing step as that can mess with the eosin's pH which will also decrease staining.
-----Original message----- From: "Hannen, Valerie" valerie.han...@parrishmed.com Date: Thu, 09 Aug 2012 09:23:26 -0500 To: histonet histonet@lists.utsouthwestern.edu Subject: [Histonet] Eosin > Hi Folks...I am hoping you all give me a little help. Our Pathologists are complaining about our Eosin on the H&E's being weak. The funny thing is, is that it can go one > > day to the next...one day it looks great...the next it is weak!! > > I have already done some experimenting with...1) time tissue spends in Eosin.... 2) making sure that the alcohols after Eosin are the properconcentrations....3) > > reducing the time that the tissues spends in the alcohols atfer Eosin... I have even gone as far as 4) increasing the rinse time in water after the decolorizing and bluing > > steps. 5) I have checked the pH of the water as well. > > Any help and suggestions would greatly appreciated!! > > Thanks Gang!! > > Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) > Histology Section Chief > Parrish Medical Center > 951 N. Washington Ave. > Titusville, Florida 32976 > Phone:(321) 268-6333 ext. 7506 > Fax: (321) 268-6149 > valerie.han...@parrishmed.com > > > > > > > > * > > > "This email is intended solely for the use of the individual to > whom it is addressed and may contain information that is > privileged, confidentialor otherwise exempt from disclosure > under applicable law. If the reader of this email is not the > intended recipient or the employee or agent responsible for > delivering the message to the intended recipient, you are > hereby notified that any dissemination, distribution, or > copying of this communication is strictly prohibited. If you > have received this communication in error, please immediately > delete this message. Thank you" > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Brendal C. Finlay, HT (ASCP) West Florida Medical Center Clinic brendal.fin...@medicalcenterclinic.com phone - 850.474.8758 fax - 850.474.8584 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
