Dear Histonetters, Has anyone had success with Dako's Her 3 antibody on paraffin sections? I am looking for a protocol to work on a DAKO autostainer or Ventana XT. I have tried the high ph Dako retrieval suggested but I am not getting much staining and I have ran a multiple tissue control block with many of the suggested controls in the block. I am going to try again using a different antibody diluent because the one I am using may not be compatible with the antibody. Does anyone have any other suggestions. ( I have tried 1:50 for 60 min primary antibody incubation)
Elaine Dooley HTL Shands Teaching Hospital Gainesville FL 352-265-0111 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
