Elaine We have had success with the Dako antibody on human tumors in mouse xenograft tissue, we used pH9 for retrieval. Since we were working in a mouse background we utilized a mouse on mouse detection system from MaxVision it worked well in our hands at 1:10 dilution for 30 minutes at room temp on the dako stainer.
Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Dooley, Elaine O. Sent: Thursday, September 20, 2012 12:27 PM To: histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] Her 3 Dear Histonetters, Has anyone had success with Dako's Her 3 antibody on paraffin sections? I am looking for a protocol to work on a DAKO autostainer or Ventana XT. I have tried the high ph Dako retrieval suggested but I am not getting much staining and I have ran a multiple tissue control block with many of the suggested controls in the block. I am going to try again using a different antibody diluent because the one I am using may not be compatible with the antibody. Does anyone have any other suggestions. ( I have tried 1:50 for 60 min primary antibody incubation) Elaine Dooley HTL Shands Teaching Hospital Gainesville FL 352-265-0111 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
