Our protocol on Ventana Benchmark Ultra: CC1 mild (ca. 30 min high pH retrieval), 1:40 for 32 min at 37°C.
We use this special lot since 2006, now aliquotted and frozen. I am sure (or hope), that we've got a positiv result, when introducing it. Unfortunately we have no positive controls available. So I'm not convinced of the reliability today. :( It will be challenging to establish the new antibody. I found a SV40 antibody from Cellmarque, but I'm not sure, if this one is the relevant one for renal transplants. Publications always talk about BK-Virus. Thanks Gudrun -----Ursprüngliche Nachricht----- Von: Richard Cartun [mailto:rcar...@harthosp.org] Gesendet: Donnerstag, 04. Oktober 2012 18:52 An: gu.l...@gmx.at Betreff: Re: [Histonet] Polyoma for renal transplantation I forgot to ask if you were able to get the Novocastra antibody to work on FFPE tissue? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-2204 Fax >>> "Gudrun Lang" <gu.l...@gmx.at> 10/4/2012 11:36 AM >>> Hi! Can anyone recommend a good Polyoma antibody usefull for testing with renal transplatation. Novocastra's Polyoma JC/BK is not available now. Thank you Gudrun Lang _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet