We also use Calbiochem on the Ventana Ultra and XT platforms.
-----Original Message-----
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tony Henwood
(SCHN)
Sent: Thursday, October 04, 2012 7:50 PM
To: 'gu.l...@gmx.at'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Polyoma for renal transplantation
Hi Gudrun,
We use the Calbiochem antibody Cat No DP02 on the Bond Platform (see below)
We originally compared it to the Novocastra's antibody and found it superior
ANTIGEN NAME: BK Virus
OTHER NAMES: SV40 T Antigen
Clone: PAb416
Isotype: IgG2a
DESCRIPTION: Monoclonal antibody to the ~94 kDa SV40 large T antigen.
STORAGE CONDITIONS: 4-8oC undiluted
SUPPLIER: Calbiochem DP02
PROCEDURE:
Methodology: HRP-POLYMER Working Dilution: Bond: 1/700
(ER2)
Special Conditions: EDTA Heat induced epitope retrieval required
CONTROL TISSUE: BK Virus Control
Inbuilt Controls
CLINICAL SIGNIFICANCE:
In immunocompromised patients, BK can cause a morphologically manifest renal
infection with cytopathic signs and functional impairment. In native and
transplanted kidneys, BK virus is found in areas of interstitial nephritis.
REFERENCES:
Nickeleit et al (1999) "Polyomavirus Infection of Renal Allografts" J Am Soc
Nephrol 10: 1080-1089.
Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory
Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead
NSW 2145, AUSTRALIA
-----Original Message-----
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang
Sent: Friday, 5 October 2012 1:37 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Polyoma for renal transplantation
Hi!
Can anyone recommend a good Polyoma antibody usefull for testing with renal
transplatation.
Novocastra's Polyoma JC/BK is not available now.
Thank you
Gudrun Lang
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