There are knife sharpening services Sturkey and Dorn and Hart are two that come to mind. You can also find refurbished disposable blade holders and buy disposable blades, the blade holder you get will determine if you use low profile or high profile blades.
Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting, LLC 40864 Arkansas Ave Bennett, CO 80102 Phone: 303-644-4538 Fax: 720-859-4110 pru...@ihctech.net -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Massimo Sent: Sunday, November 11, 2012 2:45 AM To: Jon Krupp; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Microtome knives I prefer to sharpen my microtome knives by myself by hand. I have a vintage Cambridge Rocking Microtome and despite its age it works very well. Sharpening is a time consuming for the first time, it's depends on the conditions of the blade edge. Once you have a nice cutting profile its maintenance it's quite easy and it takes a few minutes by stroking the knife on a flat glass with oil and a bit of aluminium oxide powder (3 -1 micron grits). For me sharpening and honing of a microtome knife has became a secondary "hobby". A solid knife has the advantage, compared to a disposable blade, to be liable to less vibrations. Kind Regards, Massimo Tosi "A humble Chemical Engineer who loves Histology" ________________________________ Da: Jon Krupp <jkr...@deltacollege.edu> A: Histonet@lists.utsouthwestern.edu Inviato: Venerdì 9 Novembre 2012 19:49 Oggetto: [Histonet] Microtome knives Greetings I need some advice regarding microtome knives. I am not histo tech, I did all my sectioning in a plant research lab, but now I find myself needing to learn more about histo type methods. We have microtomes, AO 820's, and we have a bunch of donated knives. I need advice about whether it would be better to find a knife sharpener and use the microtome knives we have, or check into getting a disposable knife holder. When I was sectioning, we just used a simple razor blade holder. Now I see references to high profile and low profile blades and holders, and I don't know the difference. Anyone willing to help me out? Thanks Jon Jonathan Krupp Delta College 5151 Pacific Ave. Box 212 Stockton, CA 95207 209-954-5284 jkr...@deltacollege.edu Find us on Facebook @ Electron Microscopy at SJ Delta College _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet