I might also add that Delaware Diamond Knives (DDK) also sells and sharpens microtome knives! Jack
Jack L RatliffRatliff Histology Consultants, LLC317-281-1975 > From: pru...@ihctech.net > To: max_histo...@yahoo.it; jkr...@deltacollege.edu; > Histonet@lists.utsouthwestern.edu > Date: Sun, 18 Nov 2012 07:50:11 -0700 > Subject: RE: [Histonet] Microtome knives > CC: > > There are knife sharpening services Sturkey and Dorn and Hart are two that > come to mind. You can also find refurbished disposable blade holders and > buy disposable blades, the blade holder you get will determine if you use > low profile or high profile blades. > > Patsy Ruegg, HT(ASCP)QIHC > Ruegg IHC Consulting, LLC > 40864 Arkansas Ave > Bennett, CO 80102 > Phone: 303-644-4538 > Fax: 720-859-4110 > pru...@ihctech.net > > -----Original Message----- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Massimo > Sent: Sunday, November 11, 2012 2:45 AM > To: Jon Krupp; Histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Microtome knives > > I prefer to sharpen my microtome knives by myself by hand. > I have a vintage Cambridge Rocking Microtome and despite its age it works > very well. > Sharpening is a time consuming for the first time, it's depends on the > conditions of the blade edge. > Once you have a nice cutting profile its maintenance it's quite easy and it > takes a few minutes by > stroking the knife on a flat glass with oil and a bit of aluminium oxide > powder (3 -1 micron grits). > For me sharpening and honing of a microtome knife has became a secondary > "hobby". > A solid knife has the advantage, compared to a disposable blade, to be > liable to less vibrations. > > Kind Regards, > Massimo Tosi > > > "A humble Chemical > Engineer who loves Histology" > > > > > > ________________________________ > Da: Jon Krupp <jkr...@deltacollege.edu> > A: Histonet@lists.utsouthwestern.edu > Inviato: Venerdì 9 Novembre 2012 19:49 > Oggetto: [Histonet] Microtome knives > > Greetings > > I need some advice regarding microtome knives. I am not histo tech, I did > all my sectioning in a plant research lab, but now I find myself needing to > learn more about histo type methods. > > We have microtomes, AO 820's, and we have a bunch of donated knives. I need > advice about whether it would be better to find a knife sharpener and use > the microtome knives we have, or check into getting a disposable knife > holder. > > When I was sectioning, we just used a simple razor blade holder. Now I see > references to high profile and low profile blades and holders, and I don't > know the difference. > > Anyone willing to help me out? > > Thanks > > Jon > > Jonathan Krupp > Delta College > 5151 Pacific Ave. > Box 212 > Stockton, CA 95207 > 209-954-5284 > jkr...@deltacollege.edu > > Find us on Facebook @ > Electron Microscopy at SJ Delta College > > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
